| a-Lipoic Acid, Coenzyme Q10 is caused by medicine in recent years, scholars attach great importance to scholars and kinematics of the material, In the past, a Lipoic Acid stay in their understanding of the impact of energy metabolism and the treatment of some diseases. Understanding of coenzyme Q10 remains on the treatment of cardiovascular disease. However, there is oxidation of both more and more attention. As the high-intensity exercise makes the body produce large amounts of free radicals, and thus lead to lipid peroxidation, leading to changes in cell structure and function, so that important cell organelle damage to mitochondrial membrane structure, function decline, ATP synthesis decline, can not meet the needs of body movement, thus contributing to generate even exhaustive exercise-induced fatigue. Thus,exogenous antioxidants added to protect the structure and function of mitochondria from damage, enhance cellular antioxidant capacity, improve exercise capacity has an important significance.These experiment male Kunming mice by giving additional the mixture of a lipoic acid and coenzyme Q10 mixture for 4 weeks to observe this two mixture on endurance exercise in mice antioxidant effects.Attempt to observe the mixture ofα-lipoic acid and coenzyme Q10 in the anti-role of exercise-induced fatigue. Method: keep the 24 male Kunming mice were randomly divided into three groups:quiet group (C),exercise group (T),exercise and giving medicine group (TA).This three groups were fed in normal, sports medicine group (TA) before exercise 1 hour daily intragastrically 10mg/100g.d ofα-lipoic acid and mixed coenzyme Q10 13mg/100g.d of antioxidant nutrients, Exercise group (T) and exercise treatment group (TA) for 4 weeks without weight-bearing endurance swimming training increased 6 times a week. After 4 weeks,a quiet group(C), exercise group (T), exercise and giving medicine group (TA) drawn immediately after exhaustive exercise, take mouse liver and quadriceps measured GSH, MDA contents and SOD activity.The results showed that:After exhaustive swimming,(1)endurance exercise in mice after exhaustive exercise, liver and skeletal muscle SOD activity in the exercise and added antioxidants had a significantly reduced; liver and skeletal muscle MDA content was significantly higher than exercise group added antioxidants;liver GSH was significantly lower than the exercise group for antioxidant nutrients, indicating antioxidant nutrients to improve the athletic ability to play an active role in promoting.(2) SOD activity levels of mice:1)on liver SOD activity levels in the exercise group and sedentary control group was significant difference between (p<0.05);sports supplement a-lipoic acid and coenzyme Q10 group and between the quiet control group was significant difference (p<0.01);sports supplement a-lipoic acid and coenzyme Q10 group and exercise group SOD activity was significantly different (p<0.05).2) skeletal muscle SOD activity levels, exercise added a-lipoic acid and coenzyme Q10 group and between the quiet control group was significant difference (p<0.01);the exercise group and exercise to add a-lipoic acid and coenzyme Q10 group was significantly different (p<0.05).(3)MDA content in mice:1)liver MDA content, sports supplement a-lipoic acid and coenzyme Q10 group and exercise group were significantly different between (p<0.05);sports supplement a-sulfur bitterness and the coenzyme Q10 group and quiet group were significantly different between groups (p<0.05).2) MDA content in skeletal muscle, the exercise group and sedentary control group were significantly different between (p<0.05);sports supplement a-lipoic acid and coenzyme Q10 group and exercise group were significantly different between (p<0.05); the movement to add a-lipoic acid and coenzyme Q10 group and sedentary control group mean MDA content were significantly different (p<0.01).(4) GSH content of mice:1) GSH content of the liver, the exercise group and sedentary control group were significantly different between (p<0.05); sports supplement a-lipoic acid and coenzyme Q10 group and sedentary control group was significant difference (p<0.01);the movement to add a-lipoic acid and coenzyme Q10 group and exercise group were significantly different (p<0.01).2) GSH content of skeletal muscle, sports supplement coenzyme Q10 and a-lipoic acid group and quiet were significantly different between groups (p<0.05).But the campaign added a-lipoic acid and coenzyme Q10 group and training were not significantly different between groups.The result show that:(1)Quiet endurance training group and after exhaustive exercise in mice, SOD activity in liver and skeletal muscle antioxidant nutrients than the sports supplement group were significantly ecreased; liver and skeletal muscle MDA content was significantly higher than exercise group added antioxidants;liver GSH content was significantly lower than in the movement to the group of antioxidant nutrients;exhaustive exercise time was significantly lower than the exercise group added antioxidants,indicating production of oxygen free radicals exhaustive exercise on liver and skeletal muscle mitochondria in mice resulted in varying degrees injury, but also illustrates the antioxidant nutrients in reducing free radicals damage to mitochondria play a positive protective effect.(2) This experiment used mice to endurance sports supplement a-lipoic acid and coenzyme Q10 mixture, liver and skeletal muscle of mice SOD activity significantly increased, MDA content was significantly lower liver GSH content was significantly higher.Note added a-lipoic acid and coenzyme Q10 mixture in reducing exercise-induced lipid peroxidation in vivo,scavenging oxygen free radicals, protect the mitochondrial membrane stability, and eliminate fatigue played an important role.(3)Movement combined with added a-lipoic acid and coenzyme Q10 mice swimming time compared with endurance exercise group and quiet group was significantly prolonged, indicating movement combined with added a-lipoic acid and coenzyme Q10 reduces the level of lipid peroxidation in mice, increased exercise capacity in mice.(4) In conclusion, exercise, adding a-lipoic acid and coenzyme Q10 can reduce exercise-induced lipid peroxidation. Generated by the movement of the body remove free radicals,protect the mitochondrial membrane, anti-fatigue play a crucial role.
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