Study On The Effect And Mechanism Of Hyperbaric Oxygen On Anti-exercise-induced Fatigue In Rats

Recovery of exercise-induced fatigue is hotspot problem in sports medicine research. Severe hypoxia is one of the prominent characteristics of the exercise-induced fatigue of body, hyperbaric oxygen can improve absorb and use of the oxygen, so that organizations obtain large amounts of oxygen and improve the condition of systemic hypoxia. In recent years, a growing number of studies suggest that there are broad prospects for development of hyperbaric oxygen in exercise-induced fatigue and rehabilitation, but the body specific morphology and metabolic changes on hyperbaric oxygen anti-exercise-induced fatigue and its fatigue mechanism remains to be further explored.Objective:1. To establish animal model of chronic exercise-induced fatigue in SD rats.2. To study the relationship of blood lactate and blood urea nitrogen and exercise-induced fatigue in rats.3. To study the morphology structure changes of the liver, skeletal muscle and kidney tissue in exercise-induced fatigue rats.4. To study the effect of exhaustive time of hyperbaric oxygen on rats.5. To study the effect of content of blood lactate and blood urea nitrogen of hyperbaric oxygen on rats.6. To study the effect of structure of the liver, skeletal muscle and kidney tissue of hyperbaric oxygen on rats.7. To study the effect of Malondialdehyde (MDA) content and activity of Superoxide Dismutase (SOD) of Serum、liver and skeletal muscle of hyperbaric oxygen on rats.Method:1. To establish animal model of chronic exercise-induced fatigue in SD rats for eight weeks weight loading swimming to exhaustion. And compare the change of swimming time after hyperbaric oxygen.2. To detect the content change of blood lactate and blood urea nitrogen of the control group, fatigue model group and fatigue of hyperbaric oxygen group by automatic biochemical analyzer.3. To analysis the morphology structure changes of liver、skeletal muscle and kidney tissue of the control group, fatigue model group and fatigue of hyperbaric oxygen group by HE staining and optical microscopy.4. To detect the change of MDA content and SOD activity of the control group, fatigue model group and fatigue of hyperbaric oxygen group by spectrophotometer.Result:1. The animal model of chronic exercise-induced fatigue in SD rats had been established successfully. Compared with the control group, the blood lactic acid and blood urea nitrogen content of fatigue model group increased significantly, there are significant difference between them (P<0.01). And appeared different degrees of injury in the live、skeletal muscle and kidney tissue of fatigue model group.2. Compared with the fatigue model group, the swimming exhaustive time of fatigue of hyperbaric oxygen group significantly prolonged, there was significant difference between them (P<0.05).3. The content of blood lactic acid of control group、fatigue model group and fatigue of hyperbaric oxygen group had significant differences (P<0.01), a further comparison of two group found that, compared with the control group, the blood lactic acid content of fatigue model group and fatigue of hyperbaric oxygen group were significantly higher (P<0.01); while the blood lactic acid of fatigue of hyperbaric oxygen group significantly lower than the fatigue model group (P<0.01); the content of blood urea nitrogen of control group、fatigue model group and fatigue of hyperbaric oxygen group also had significant differences (P<0.05), a further comparison of two group found that, the blood urea nitrogen content of fatigue model group and the control group、fatigue of hyperbaric oxygen group had significant difference (P<0.01), compared with the fatigue model group, while the blood urea nitrogen content of the fatigue of hyperbaric oxygen group was decreased, but not statistically significant (P>0.05).4. Compared with the fatigue model group, the structure injury situation on liver、skeletal muscle and kidney tissue of the fatigue of hyperbaric oxygen group has improved.5. The SOD activity on serunm liver and skeletal muscle of control group、fatigue model group and fatigue of hyperbaric oxygen group were significant differences (P<0.01). A further comparison of two group found that, the SOD activity of serum control group was significantly higher than the other two groups (P<0.01), While the fatigue of hyperbaric oxygen group increased significantly than the fatigue model group (P<0.01); the SOD activity of liver and skeletal muscle in control group was significantly higher than the other two groups (P<0.01), while the fatigue of hyperbaric oxygen group were increased than the fatigue model group, but not statistically significant (P>0.05).6. The MDA levels on serum and skeletal muscle of control group、fatigue model group and fatigue of hyperbaric oxygen group were significant differences (P<0.01). A further comparison of two group found that, the content of MDA in control group was significantly lower than the other two groups (P<0.01),While the fatigue of hyperbaric oxygen group decreased significantly than the fatigue model group (P<0.01); the MDA levels on liver of control group、fatigue model group and fatigue of hyperbaric oxygen group were significant differences (P<0.05). A further comparison of two group found that, the content of MDA in the fatigue model group was significantly higher than the other two groups (P<0.01), although the fatigue of hyperbaric oxygen group somewhat lower than fatigue model group, but no statistical significance (P>0.05).Conclusion:1. The experiment confirms that the animal model of chronic exercise-induced fatigue was successfully established from the content of blood lactate and blood urea nitrogen as well as the liver, kidney, skeletal muscle morphology injury two sides, provides a good animal model of exercise fatigue for the follow-up experiment.2. The experiment was confirmed by hyperbaric oxygen with good recovery effect to exercise-induced fatigue.3. The experiment certified hyperbaric oxygen anti-exercise-induced fatigue relate to the SOD activity and MDA content of rat serum, liver and skeletal muscle tissue, prompting HBO anti-exercise-induced fatigue mechanism may be related to the generation and removal of oxygen free radical, and its specific molecular mechanisms need further research.