Characteristics Of Intestinal Flora Diversity And Identification Of Driving Strains And Prediction Of Function In Colorectal Cancer Patients

Objective Based on the results of high-throughput sequencing,to compare the diversity of intestinal microflora in patients with colorectal cancer,to explore the role of core driving bacteria in the pathogenesis and development of colorectal cancer,and to predict and further analyze the functional pathway of intestinal microflora.Methods Through the second generation of highthroughput sequencing technology,the subjects of study were the patients who underwent r OTUine colonoscopy.Stool samples were collected and divided into three groups: healthy control group,adenomatous polyp(precancerous lesion stage)group and colorectal cancer group.Sample DNA was extracted,16 Sr RNA Gene was amplified and V3,V4 region PCR was performed Amplification,to analyze the species diversity of fecal samples,at the same time to compare the structure and composition of different stages of cancer patients,to analyze the species and abundance of bacteria in each sample,to identify the core driving bacteria in the development process of colorectal cancer from the ecological point of view,and to further predict the function of the analysis.Results There were significant differences in the intestinal microbial structure and species diversity between the colorectal cancer patients and the control group.The intestinal microflora in the colorectal cancer group had lower species diversity than that in the control group.With the development of colorectal cancer,the diversity of intestinal flora decreased significantly in the later stage of pathological stage.There was significant difference between the intestinal flora structure of healthy control group and colorectal cancer patients,but there was no significant difference between the intestinal flora structure of patients with different pathological stages.Eight potential driving strains were screened by relative abundance analysis,which were highly correlated with the pathological development of colorectal cancer.Conclusion The changes of intestinal microflora in patients with colorectal cancer may affect the development of colorectal cancer.Comparing the changes of intestinal microflora structure is helpful to study the differences of internal microecological environment in the development of colorectal cancer.To identify the driving bacteria related to the development of pathological stages and confirm that the change of the abundance of these bacteria is closely related to the occurrence and development of colorectal cancer,which is conducive to the use of related microbial markers as early screening markers for colorectal cancer.