Study On The Regulating Effect Of Modified Huanglian Decoction On Gastrointestinal Flora In Rats With Chronic Atrophic Gastritis

Background:Chronic atrophic gastritis(CAG)is a common gastrointestinal disease.It is characterized by atrophy of mucosal epithelium and glands,and thinning of mucosa.Chronic atrophic gastritis is closely related to the occurrence of gastric cancer and is recognized as a precancerous lesion of gastric cancer.Modified Huanglian Decoction is an effective prescription for treating chronic atrophic gastritis in many years of clinical application.The mechanism of this prescription in treating chronic atrophic gastritis is not clear,and its regulating mechanism on the intestinal flora has not yet been clarified.The in-depth discussion of its mechanism of action is clinical Application provides a scientific basis.Objective:To replicate chronic atrophic gastritis model in Sprague Dawley(SD)rats,and observe the effect of Modified Huanglian Decoction on the structure of gastric mucosa in rats with chronic atrophic gastritis;Quantitative real-time-PCR(qPCR)was used to detect interleukin 1β(Interleukin,IL-1β)and secretory immunoglobulin A(SIgA)of gastric mucosa in rats with chronic atrophic gastritis Influence of expression.16S ribosomal RNA gene sequencing analysis(16S rDNA)sequencing was used to study the effect of Jiawei Huanglian Decoction on the gastrointestinal flora of rats with chronic atrophic gastritis.Methods:Thirty SD rats weighing 150±20g were randomly divided into three groups using the random number table method:normal control group(NC),chronic atrophic gastritis model group(CAG group),and Modified Huanglian decoction group(MHLD)group).The CAG group and the MHLD group were alternated daily with 0.1%ammonia solution and 20 mmol/L sodium deoxycholate solution,and a model of chronic atrophic gastritis was reproduced with the starvation disorder method.The model was made for 10 weeks.The soup was administered to the stomach for 2 weeks(13.65g/KG/d),and the NC group and the CAG group were administered the same amount of saline(lml/100g/d).After the intervention,rat gastric mucosa tissues and feces were collected,and the gastrointestinal flora was detected by 16S rDNA sequencing;the quantitative real-time-PCR(qPCR)technology was used to detect the expression of IL-1β and SIgA in gastric mucosa Horizontal;paraffin sections and hematoxylin-eosin staining light microscope were used to observe the differences in gastric mucosal structure in rats.Results:Pathological observations showed that the NC group had a complete gastric mucosa structure,neatly arranged epithelial cells and glands,abundant glands,and cells It was a simple column;in the CAG group,gastric mucosa was thinned,and the glandular cells were disordered and significantly reduced;in the MHLD group,the glandular cells were arranged relatively neatly and the glandular cells were reduced less.Q-PCR results showed that IL-1β expression level in CAG group was significantly increased compared with NC group;IL-1β expression level was significantly reduced in MHLD group compared to CAG group;SIgA expression level was significantly decreased in CAG group compared to NC group;Group,MHLD group SIgA expression level increased significantly.16S rDNA sequencing results showed that in the gastric mucosa flora,compared with the NC group,there was no significant difference in the richness of the flora(ACE,Chao1)in the CAG group,and the diversity index(Shannon and Simpson)and the uniformity(J)decreased significantly;Compared with the CAG group,the MHLD group had no significant difference in richness(ACE,Chaol),and the diversity index(Shannon and Simpson)and evenness(J)increased significantly;βdiversity indicated that the NC group was significantly different from the CAG group,and MHLD The group was similar to the NC group;through analysis of LDA Effect Size,it was found that the number of Bifidobacteriaceae and Bacteroidaceae in the MHLD group was significantly increased,and that of Pseudomonadaceae was significantly lower than in the CAG group;Functional prediction shows that in the intestinal flora,alanine,aspartic acid and glutamic acid metabolism,amino sugar and nucleotide sugar metabolism,polyketose unit biosynthesis,fructose and mannose metabolism and RNA degradation in CAG Significantly decreased in the group and increased in the MHLD group;analysis of fecal intestinal flora revealed three groups richness(ACE,Chao1),diversity index(Shannon and Simpson),and evenness(J)Significant differences;β Diversity showed some differences among the three groups;LDA Effect Size analysis found that the MHLD group was significantly higher than the model group,Rikenellaceae,and Dehalobacteriaceae,Veillonellaceae,etc.,Enterococcaceae,Streptococcaceae,Pseudomonadales,etc.are significantly lower than CAG group.Conclusion:MHLD can promote CAG rats to repair gastric mucosa pathology and promote gastric mucosal repair;significantly reduce IL-1β expression level and increase SIgA expression level.MHLD can restore the diversity and flora structure of gastric mucosa,restore the function of some flora,improve some beneficial flora,and promote the restoration of gastric mucosa and intestinal flora imbalance.