Distribution Of Intestinal Flora In Patients With Polycystic Ovary Syndrome And Changes Before And After Treatment

Research purposes: Polycystic ovary syndrome(PCOS)is a common endocrine disease in women of childbearing age with unknown pathogenesis.At present,a large number of studies have found that the intestinal flora has a profound influence on the development of polycystic ovary syndrome.This experiment analyzes the distribution characteristics of the intestinal microbes in patients with PCOS and the changes in the flora before and after treatment,and explores the relationship between PCOS and the intestinal flora.Materials and methods: According to the inclusion criteria,16 patients with polycystic ovary syndrome who were diagnosed for the first time and had not yet started treatment were collected,and 6 normal people were collected as a control group.Nine patients treated with Yasmin for 3 months or more(including 3 months)were used as the treatment group.The fecal DNA was extracted,16 Sr DNA was amplified by PCR,and a sequencing library was established.The sequencing was performed on the ION PROTON sequencing platform.The sequencing data was analyzed by QIIME2 software,and the structure of the flora and the differences between the groups were analyzed using the relevant R package.Results: 1.Compared with the healthy control group,the alpha diversity of the intestinal flora of the PCOS group was reduced,but there was no significant statistical difference.2.There was no statistical difference in β diversity between healthy control group and PCOS group.3.There was no statistical difference in the α diversity and βdiversity of the intestinal flora between the Yasmin treatment group and the PCOS group.4.The level of phylum classification,the abundance of Actinobacteria(P=0.013)and Proteobacteria(P=0.039)in the PCOS group was lower than that of the control group,and there was no significant difference between Bacteroides and Firmicutes(P>0.05).5.The abundance of Betaproteobacteria,Clostridiaceae,Bacteroidaceae,Rikenellaceae,Bacteroides,Clostridium,Eubacterium,Granulicatella,Megamonas,Ruminococcus,Alistipes-finegoldii,Alistipes-putredinis,Bifidobacterium-longum,Ruminococcus-bromii,and Veillonella-dispar were lower than those of the control group(P<0.05).6.The abundance of Desulfovibrionales,Odoribacteraceae,Rikenellaceae,and Alistipes-putredinis in the Yasmin treatment group is higher than that in the PCOS group(P<0.05),the abundance of Alphaproteobacteria,Lachnospiraceae,Ruminococcus,Bifidobacterium-longum,Ruminococcus-bromii,Bacteroides-uniformis was lower than that of PCOS group(P<0.05).7.The metabolic abnormalities of patients with polycystic ovary syndrome are related to their intestinal flora.Conclusion: 1.There are different bacteria in the PCOS group and the control group,and the different bacteria are related to the metabolism of the host,suggesting that the intestinal flora may be involved in the occurrence and development of polycystic ovary syndrome.Monitoring the patient’s intestinal flora may be a new indicator of disease diagnosis.2.There are different bacteria between the treatment group and the PCOS group,and the difference between the bacteria and the metabolism of the treatment group is related,suggesting that the improvement of the clinical characteristics of the treatment group is related to the change of the host flora,and changing the host’s intestinal flora characteristics may become more treatment options.A new target for cystic ovary syndrome.