Effects Of 3-NPA Oxidative Stress On Reproductive Function Of Parental Mice And Their Offspring

Stress in work and life makes people always in a state of continuous stress,which is likely to cause oxidative damage and affect human health.On the other hand,under the management mode of modern animal husbandry,serious oxidative stress is caused to livestock,resulting in individual growth and development blocked,organ function weakened,and ultimately reduce the quality of livestock products.It is necessary to study whether the oxidative stress injury in parents affects the function of offspring.At present,the research on the damage of parents’ oxidative stress to the offspring’s reproductive function only involves the influence of mother on offspring,while the influence of father on offspring is rarely reported.Moreover,the research on the influence of oxidative stress of parents on reproductive function of offspring has not been reported.Moreover,the research on the effect of oxidative stress of parents to the reproductive function of offspring has not been reported.Therefore,in this experiment,the oxidative stress model mice were induced by 3-nitropropionic acid.This study was designed to single or both of the parents oxidative stress responsed groups.The first generations were obtained by natural mating,and subsequently,the offsprings in the same group were selfed mating again.This study was aimed to explore whether the reproductive capacity of offspring is related to unilateral oxidative stress or both oxidative stress of parents,which will provide a basis for related research.The experimental methods are as follows: 5-week-old healthy Kunming mice were induced to be oxidative stress model mice by 3-nitropropionic acid.Methods:model mice were injected intraperitoneally with 12.5mg 3-nitropropionic acid per kg body weight for 7 days,twice a day.Four mating groups were designed: normal male and normal female mating group(NM-NF),normal male and model female mating group(NM-MF),model male and normal female mating group(MM-NF),model male and normal female mating group(MM-NF)and model male and model female mating group(MM-MF).The reproductive traits of parents and offspring were observed,and analyzed by SPSS18.0 Statistical software.The results showed that: in parents,1.testicular index: model male mice were significantly smaller than normal male mice(P < 0.05);ovarian index: model female were significantly smaller than normal female(P < 0.05);uterine index: model female and normal female had no significant difference(P > 0.05);2.Mating-pregnancy time:there was significant difference between normal mating group and NM-MF and MM-NF groups(P < 0.05).There was extremely significant difference between normal mating group and MM-MF group.There was no significant difference between NM-MF and MM-NF group(P > 0.05),however they were significantly different with MM-MF group(P < 0.05);3.Number of litter size after mating 30 days:the normal mating group was significantly different with NM-MF and MM-NF group(P < 0.05)and there was extremely significant difference between normal mating group and MM-MF group(P < 0.01).There was no significant difference between NM-MF and MM-NF group(P > 0.05),but they were significantly higher than MM-MF group(P < 0.05);4.Liter size: the normal mating group was significantly different with NM-MF and MM-NF group(P < 0.05),there was extremely significant difference between normal mating group and MM-MF group(P < 0.01).No significant difference was found between NM-MF and MM-NF group(P > 0.05),but they were significantly higher than MM-MF group(P < 0.05);5.Kidding rate: there was no significant difference among the four groups;6.Survival rate of weaning at6.3 weeks old: there was no significant difference among the four groups.In the offspring,1.Ovary index,testis index: there was no difference among normal group,NM-MF and MM-NF group,however they were significantly different with MM-MF group(P < 0.05);2.There was no significant difference in uterus index,spleen index,kidney index,liver index,heart index and spleen index between different groups(P>0.05);3.There was no significant difference in body weight between different group at 3,7,21 days and 4 weeks age(P > 0.05);4.Mating-pregnancy time: there was no significant difference between normal mating group and NM-MF and MM-NF groups(P >0.05),while they were significantly different with MM-MF group(P < 0.05);5.Number of litter size after mating 30 days:there was no significant difference among normal mating group and NM-MF and MM-NF group,however,they significantly higher than MM-MF group respectively(P < 0.05);6.Liter size: there was no significant difference among normal mating group and NM-MF and MM-NF group,they all significantly higher than MM-MF group(P < 0.05);7.Kidding rate: there was no significant difference among the four groups;8.Survival rate of weaning at 6.3 weeks old: there was no significant difference among the four groups.Conslusion:1.In parents,3-nitropropionic acid can inhibit the growth and development of testis and ovary;2.In parents,as long as one mate is exposed to 3-nitropropionic acid oxidative stress,the duration of successful pregnancy will be significantly prolonged and the number of births will be significantly reduced.And the influence of parents is the same.If both parents are exposed to oxidative stress,the situation will be aggravated;3.The offspring whose parents were exposed to oxidative stress significantly prolonged the successful pregnancy time and significantly reduced the number of births,but the reproductive function of the offspring whose parents were unilaterally exposed to oxidative stress can return to normal.