| The purpose of this study is to reveal the therapeutic effect of glutamine enhanced parenteral nutrition on canine enteritis and provide reference for clinical application of glutamine fortified parenteral nutrition in veterinary medicine.In this experiment,six rabbits were randomly divided into the experimental group and the control group.The enteritis model was established by using 1%acetic acid and distilled water enema respectively.The results were evaluated by clinical symptoms and pathological observation.The model was randomly divided into three groups:group Ⅰ(normal saline),group II(glutamine),group III(full nutrient solution)and group Ⅳ(glutamine and full nutrient solution)on the basis of conventional symptomatic antibiotic treatment.Before and five days after the experiment,clinical symptom observation,blood routine analysis,biochemical analysis and enzyme-linked immunosorbent assay(ELISA)were used to determine disease activity index(DAI),blood routine index,ALT,AST,TP,ALB,BUN,CRE,TRIG,GLU and D-lactate content.The experimental results obtained from animal experiments were directly applied to the clinical treatment of canine enteritis diseases,and the therapeutic effect was observed to conduct clinical application investigation.The investigation was divided into canine parvovirus infection enteritis and non canine parvovirus infection enteritis for statistical analysis.The treatment effect was compared with that of the cases of the same disease without application.The cure rate and average treatment days were adopted as the main index of evaluation.The results showed that:compared with the control group,the clinical symptoms and pathological changes of the experimental group were obvious,and the enteritis model of the rabbit was successfully established.Compared with statistics before treatment,DAI in group Ⅳ decreased significantly,lower than that in other three groups(P<0.05);the number of leukocytes in group Ⅳ was higher than that in other three groups(P<0.05);the number of erythrocytes and the content of hemoglobin in group Ⅲ and group IV were higher than those in group Ⅰ and group Ⅱ(P<0.05);the level of platelet among all groups rose and the level in group Ⅲ and group Ⅳ was lower than that in group Ⅰ and group Ⅱ(P<0.05).There were no significant differences in AST and ALT among all groups before and after treatment.After treatment,ALB and TP of group Ⅰ were lower than those of other three groups(P<0.05);CRE and BUN of group Ⅲ and Ⅳ were higher than those of group Ⅰ and group Ⅱ(P<0.05);TRIG and GLU of group Ⅲ and group Ⅳ were higher than those of group Ⅰ and group Ⅱ(P<0.05).The content of D-LA in group Ⅳ was lower than that in the other three groups(P<0.05).The results of clinical application investigation showed that the cure rate of glutamine intensive nutrition therapy was higher than that of non application cases;the average time spent in treating canine parvovirus infection enteritis was shorter with the application of glutamine intensive nutrition than that without it(P<0.05).In conclusion,glutamine enhanced parenteral nutrition can alleviate the clinical symptoms,meet the nutritional needs of the body,regulate the immune function,and repair the intestinal mucosal barrier,which can be used to assist the treatment of enteritis.The canine clinical effect is good,which provides a scientific basis for the research,promotion and application of veterinary drugs. |
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