Study On The Application Of Two Preservation Methods To Phialocephala Sp.,an Endophytic Fungi Of Blueberry

In this study,the blueberry endophytic fungi 9-8(Phialocephala sp.)isolated and purified from Vaccinium uliginosum in Daxinganling were taken as the research object.Continuous Subculture Preservation was used as controls,by testing and analyzing the differences in vigor(average growth rate of colonies,IAA content in culture medium,colonization rate,etc.),the two preservation methods' effect of Immobilized Granule Preservation and Filter Paper Preservation at 4 ? and room temperature and Agarslantculture-medium Preservation were evaluated.It aims to find a simple and stable way to preserve blueberry endophytic fungi for a long term.The results show:(1)The six preservation methods used in this study could maintain the growth vitality of 9-8 of blueberry endophytic fungi within the preservation period of 4 months and the ability to secrete IAA and of colonization on " Legacy " blueberry tissue culture seedlings within the preservation period of 3 months.(2)The preservation methods tested had a significant effect on the average growth rate of colonies:During the preservation period of 0 to 4 months,mean of the average growth rate of colonies Agarslantculture-medium Preservation,Continuous Subculture Preservation,Filter Paper Preservation at4 ?,Immobilized Granule Preservation at 4 ?,Filter Paper Preservation at room temperature and Immobilized Granule Preservation at room temperature was 2.91mm/d,2.83mm/d,2.64mm/d,3.04mm/d,2.36mm/d and 2.69mm/d.The fluctuation range was 0.62mm/d,0.83mm/d,0.70mm/d,0.32mm/d,1.51mm/d and 2.60mm/d.Among them,Immobilized Granule Preservation at 4? had the largest mean and the smallest fluctuation range and had the best effect on vigor of fungi.(3)The preservation methods tested had a significant effect on IAA content in culture medium: During the preservation period of 0 to 3 months,mean of IAA content in culture medium of Agarslantculture-medium Preservation,Continuous Subculture Preservation,Filter Paper Preservation at 4 ?,Immobilized Granule Preservation at 4?,Filter Paper Preservation at room temperature and Immobilized Granule Preservation at room temperature was 1.72mg/L,2.13mg/L,1.76mg/L,0.92mg/L,1.61mg/L and 0.90mg/L.The fluctuation range was 2.69mg/L,1.81 mg/L,2.46 mg/L,1.02 mg/L,2.86 mg/L and 0.93 mg/L.Among them,Continuous Subculture Preservation had the largest mean but Immobilized Granule Preservation at 4? and Immobilized Granule Preservation at room temperature had the smallest fluctuation range.(4)During the preservation period of 0 to 3 months,the difference in colonization rate using the 6 storage methods was not significant,that is,the preservation method tested had no significant difference in the ability of colonization of fungi 9-8 on " Legacy " blueberry tissue culture seedlings.(5)The comprehensive analysis believed that Immobilized Granule Preservation at 4? used in this study had the best preservation effect and is suitable for the preservation of blueberry endophytic fungi.Agarslantculture-medium Preservation and Immobilized Granule Preservation at room temperature had a good comprehensive evaluation and can also be used.Filter Paper Preservation needs further improvement.