Effects Of Endophytic Bacteria Inoculation On The Growth Of Tagetes Patula L And Remediation Ability Of Cd Contaminated Soil

Microbial-phytoremediation technology is currently the focus of extensive research.Endophytes have important ecological functions that regulate the nutrient absorption,physiological function,and resistance to contamination of plants,promote the growth of host plants,and increase the resistance of plants to cadmium.The possibility of good repair has been shown.In this paper,the main research subjects are the highly accumulative plant Tagetes patula L and its endophytes,and the research on the endophytic diversity of Tagetes patula L was conducted based on the non-culture method.Three functional strains of Alcaligenes KG3,Pseudomonas aeruginosa KG 10,and Pseudomonas aeruginosa KG 10 have been used to enhance the restoration of cadmium-contaminated soil by Tagetes patula L and the enhancement of endogenous bacteria.Maidenhair restoration of Tagetes patula L contaminated soil provides the basis for research.The main results of the survey are:1.High-throughput sequencing analysis of the bacterial flora of Tagetes patula L（leaves,stems,roots）and its soil（rhizosphere soil,non-rhizosphere soil）was performed by the non-culture method,and the predominant bacterium of Tagetes patula L was discovered.bottom.Endogenous Bacteria It belongs to Bacillus,Gematimonas,Sphingomonas,Acidobacteria,Proteobacterium,etc;For roots,stems,leaves and rhizosphere soils,non-studies on the diversity of the five niches of rhizosphere soils,The non-rhizosphere soil and the rhizosphere soil of Tagetes patula L were the smallest,the difference coefficient was 0.096,and the endogenous bacteria in the leaves of Tagetes patula L and the non-rhizosphere soil were diverse.The gender difference is the largest,and the dissimilarity coefficient is 0.821.2.Three different inoculation methods（irrigated roots,soaked roots,and irrigated roots+soaked roots）to study the effects of KG3,KY7,and KG 10 on Tagetes patula L growth,cadmium absorption,and rhizospheric soil environment.Use the.As a result,the biomass of Tagetes patula L increased significantly by root irrigation+root inoculation of KG3,KY7,KG 10,and among them,KY7 has the highest growth promoting effect,and it is fresh by inoculation of KY7 under cadmium-contaminated conditions.It was found that the weight increased.48.50 of the Tagetes patula L leaves.The fresh weight of the roots increased by 39.00%.Inoculation of KG 10 by root irrigation and root immersion can significantly increase cadmium accumulation.Inoculation of KG 10 under cadmium contamination can increase the cadmium content of roots,stems and leaves by 32.39%,36.26%and 57.23%,respectively.Rhizosphere soil pH,EC value,nitrate nitrogen content,DTPA available cadmium content,and available potassium content are positively correlated with cadmium accumulation in roots,stems,and leaves.There is a positive correlation between organic matter content and available phosphorus content in rhizosphere soils.Cadmium accumulation in various tissues of Tagetes patula L is negatively correlated,cadmium accumulation of Tagetes patula L cadmium is significantly correlated with microbial community diversity in soil environment,and cadmium accumulation capacity in maiden rhizosphere soil environment is significant.The ability of plants to accumulate cadmium,which has been shown to be associated with.3.Study the effects of KG3,KY7,and KG 10 on the physiology and biochemistry of Tagetes patula L plants using a combination of root irrigation and soaking method.Experimental results show that inoculation with endogenous bacteria significantly increases the biomass and leaf chlorophyll content of Tagetes patula L under cadmium-contaminated conditions,with catalase（CAT）,superoxide dismutase（SOD）,Peroxidase（POD）.It shows that it significantly reduced the content of malondialdehyde（MDA）in Tagetes patula L leaves.At a Cd concentration of 12 mg/kg,inoculation with KG 10 increases the fresh weight of leaves by 129.41%and the fresh weight of stems by 107.69.Fresh weight increased by 85.70%,leaf chlorophyll content,SOD activity,POD activity were 19.42%,17.80%,6.34%higher and MDA content decreased by 1.40%compared to the control group.As described above,the inoculation of the functional bacteria KG3,KY7,KG10 is useful to increase the biomass and cadmium accumulation of Tagetes patula L,and strengthen the drag of cadmium and improve the root soil environment.