Research On The Cell Architecture And Three-dimensional Visualization Of Carp Brain Motor Nerve Nucleus For Biological Control

With the rapid development and convergence of electronic information technology and neuroscience,a new field of science and technology has gradually emerged-animal robotics.Animal robots use animals as carriers,using biological control technology to make them move along the expected path according to human wishes and complete related work.The structure and three-dimensional model of the nucleus accumbens of the animal brain can provide precise three-dimensional coordinates and morphological parameters to support the control of animal robot movements.In this study,the histomorphological,3D visualisation and biocontrol techniques are used to control the movement of carp robots as follows:In terms of cellular architecture of the neural nuclei in the motor region of the carp brain,a three-dimensional cranial coordinate system was established based on the principle of brain stereotaxic positioning;cardiac perfusion was used to fix the in vivo brain tissue of carp;sagittal sections were prepared by continuous tissue sectioning technique and Nissler staining was performed to study the types of neuronal cytosomes and the arrangement of cytosome groups in nuclei VI and VII.In the 3D visualisation of neuronal clusters in carp brain motor areas,continuous tissue sections with reference pin tracts were digitally acquired,pre-processed using Matlab,then aligned using Image J,and finally segmented and 3D reconstructed using Mimics software to determine the spatial distribution and morphological characteristics of neuronal clusters in brain motor areas.In terms of the design and use of the carp robot brain electrode adapter device,the load capacity of the carp under different load modes was studied in the combined water maze,and the loading method of different devices was determined;for the problem of fixing the brain electrode of the carp robot,the skull composition of the carp was studied.,Shape and bone structure,determine the best mounting point;in view of the brain electrode and electrical stimulation platform transfer problem,combined with the weightbearing characteristics and skull characteristics,design the brain electrode transfer device.In the area of underwater motion control of carp robots,a method for controlling the steering motion of carp based on the electrical stimulation of brain motor area VI nucleus was proposed,and the effect of different parameters of electrical stimulation on the steering behaviour of carp was quantitatively investigated through a multi-arm combined water maze combined with behavioural tests to achieve basic quantitative control of carp steering behaviour.