Study Of The Location Of Motor Area And Histology About Carp-robot Based On Electrical Stimulation

Animals are better than the bionic robots in motion flexibility, complexity and adaptability to the environment in the current study. Then regarding the animal itself as the research object which called animal robot is becoming quite a novelty these days.Carp-robot is an important component of the aquatic animal robot. Based on the study that the brain can control body movement, this paper states a way to locate the motor function areas of carps.First, we investigated the brain structure of carps from a histological perspective by making brain impregnated specimens and slice preparations. Through the production of impregnated specimens to observe brain tissue in general structure and slice preparation in microscopic structure. And then we focus on the telencephalon, midbrain, cerebellum of carp brain to position motor function areas. With the way of electrolytic lesion, we damaged several designated sites of these three regions to find out the function based on the loss behavior. Light anesthesia was induced in the carps. Then the electrophysiological method was used to detect the function of different areas of the brain and effective stimulation sites was recorded. Based on the effective sites we made an underwater experiment to observe the specific movement of waking carps by electrical stimulation and then marked the effective stimulation sites.The result of brain lesion experiment shows that the midbrain, cerebellum participate in movement control, and only damage the telencephalon showed no effect on swimming movement. The probe experiment result indicates that stimulating the telencephalon can only result in the movement of the mouth and the eyes, while, stimulating the midbrain and cerebellum can cause the tail swing and fins extending. And most of the effective stimulation sites are located in the optic tectum and most area of the cerebellum. The underwater experiment reveals that stimulating the effective sites in optic tectum, left and right sides of the cerebellum can cause carp steering, and stimulating the midline of the cerebellum can cause forward and backward movement. Marked the effective stimulation sites in the tissue and we found that they almost gathered at optic tectum, fasciculus longitudinal lateral and purkinje cell layer.Based on the experiment result, we hypothesized that the carps swimming control center is the mesencephal lateral nuclear and the purkinje cell layer is the body’s balance control center. The nerve impulse conduction pathway may be:The optic tectum receive external stimulation signal and transmit it to the mesencephal lateral nuclear and the purkinje cell layer through nerve fibers, and the mesencephal lateral nuclear and purkinje cell layer is connected through nerve fibers, both of them also send fibers to myelencephalon and then connected to spinal cord, these two centers work together to guarantee the carp swimming movement.