BSP & Rankl levels in GCF during orthodontic tooth movement

Introduction. RANKL has been shown to be upregulated in the compression side during Orthodontic tooth movement, and BSP has been shown to enhance the ability of RANKL to induce bone resorption by promoting osteoclastogenesis. Thus it is hypothesized that BSP and RANKL will work synergistically to promote bone resorption at the compression side during OTM. The purpose of this study is to use orthodontic tooth movement as a controlled setting for inducing bone resorption in areas of compression, and thus clinically assess GCF levels of BSP and RANKL.;Materials and methods. Split mouth design with one of the maxillary lateral incisors serving as experimental tooth, and the contra-lateral tooth as control tooth. Ten patients between 10 to 14 years were selected from 110 prospective patients from TUSDM department of orthodontics. Patients were planned for orthodontic treatment with edgewise multi-bracket appliances; initial leveling was done with 0.014 GAC nickel titanium archwires (GAC Sentalloy). The experimental teeth were lingually positioned, and the control teeth were not engaged in the wire the first week of treatment. GCF samples were collected from the labial surface of both lateral incisors, before application of a force, at 24 hours and 168 hours after application of a force. The Periotron 8000 and its software Periotron Professional 3.0 (Oraflow Inc, Plainview, NY) were used to quantify GCF volumes. A micro BCA kit will be used to quantify total protein, and BSP and RANKL levels will be quantified using ELISA kits. A paired t-test will be used to calculate inter and intra group differences.;Results. GCF levels of RANKL and BSP did not change significantly at 24 and 168 hours after application of orthodontic force to maxillary lateral incisor teeth in children. There was no significant correlation in the changes of RANKL and BSP in GCF at 24 and 168 hours after application of orthodontic force to maxillary lateral incisor teeth in children 10 to 14 years of age.