| Objectives: Gingival crevicular fluid (GCF) is an exudate or serum transudate, which can becollected from gingival crevice surrounding the teeth. It reflects the constituents of serum, thecellular response in the periodontium and contributions from gingival crevice. During theorthodontic treatment, the mechanical forces exert distortion of the periodontal ligament (PDL)and extracellular matrix resulting in some signal transduction that causes modifications of GCF’scomponents. The objective of this study is to investigate the expressions of receptor activator ofNF-κB ligand (RANKL) and interleukin-17A (IL-17A) in GCF, in young male and female withor without the orthodontic treatment. We also investigated the correlation between RANKL andIL-17A.Materials and methods:72healthy adolescents and young adults were divided into twogroups: the treatment group (Group A,24males,24females), and the non-treatment group(Group B,12males,12females). The allocation of1:1ratio was a better representation of thewide range of age groups. It achieves90%power to detect a pattern of RANKL and IL-17A.GCF was collected from the three sites (MESIAL, MEDIAL and DISTAL) of the maxillarycanines and then the expressions of RANKL and IL-17A were measured by enzyme-linked-immunosorbent serologic assay (ELISA).Results: The expressions of RANKL and IL-17A observed in treatment group are both higherthan on-treatment group, showing statistically significant results (P <0.05), no matter males orfemales. Their expressions in males are higher than that in females, both in Group A and GroupB (P <0.05). The pearson correlation test (1-tailed) between RANKL and IL-17A is r=0.788**.Conclusion: The amount of RANKL and IL-17A increased significantly during orthodontic treatment. They both were estrogen dependent, and also had a high positive correlation with eachother. |
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