| Long-term overdose use of GC can often lead to glucocorticoid-induced osteoporosis(GIOP),which is manifested by the destruction of bone microstructure,reduced bone content,reduced bone density,and decreased bone strength,and metabolic bone disease that is prone to fracture,Becoming the third highest secondary osteoporosis.Therefore,the need to explore new methods to increase the use of GC and reduce the incidence of GIOP is the main task of our young contemporary researchers.The prevention and treatment of OP in modern medicine is mainly to increase calcium content,estrogen,calcitonin,etc.,but the economic cost is less safe.For example: urinary stones.In recent years,the treatment effect of traditional Chinese medicine has been quite good.The concept of the theory of traditional Chinese medicine is used to differentiate and treat OP,and the overall adjustment of the traditional Chinese medicine prescription compound has achieved excellent treatment results."Su Wen · Wei Lei Lun" describes "the bone marrow of the main body of the kidney".The kidney stores essence,essence and marrow,and the marrow resides in the bone cavity to nourish the bones.The kidney essence is deficient and the bone marrow is reduced.That is,kidney deficiency often causes osteoporosis,and kidney essence determines the strength of skeletal bones.The prevention and treatment of osteoporosis is based on the theory of nourishing kidney and strengthening bones,and it is a masculine glucocorticoid that is a "pure Yang product".,Long-term use of large doses can easily lead to kidney yin deficiency,the use of Bushen Zhuanggu compound Zhuanggu tablets is based on this theoretical formula.Purposes:By studying the effect of Zhuanggu tablets on the serum biochemical indexes of secondary glucocorticoid-induced osteoporosis in rats,and the related metabolic parameters of the left femur,bone density and histological indexes of the right femoral morphology,through the Western medicine Cal Odd D contrast to clarify the possible mechanism of Chinese medicine compound Zhuanggu tablets on the regulation of bone metabolism and the prevention and treatment of osteoporosis.How good is Zhuanggu Tablet in preventing GIOP?Can the rationality and scientificity of traditional Chinese medicine theories such as "Kidney bones store spermatozoa" and "Bushen is also like bone filling".Material and methods:Forty healthy 3-month-old Wister rats(SPF-grade SD female rats)were weighed at 200 ± 20 g.7 days of adaptive breeding,changing litter every three days.Randomly divided into four groups of feeding,blank control group(group A),model control group(group B),Chinese medicine(Zhuanggu tablet)treatment group(group C)and western medicine(calcic D)control group(group D)each 10 Only,except the group A,the other groups were given dexamethasone injection 0.25 mg / 100 g intraperitoneally 2 times / week(Monday,Thursday)for modeling.Chinese medicine treatment group + Zhuanggu tablet gavage,Western medicine control group + Calci D D gavage,blank control group and model control group gavage with distilled water(10mg / kg)for 8 weeks,weighing once a week,adjusting at appropriate time Dexamethasone injection volume.After 8 weeks,the rats were dissected,blood was taken and centrifuged,and the bilateral femurs of the rats were taken.Micro-CT was used to determine the changes of bone mineral density morphology,related bone metabolism parameters,HE staining to observe bone tissue morphology,biochemical determination of blood phosphorus,calcium and Serum alkaline phosphatase and osteocalcin were detected by ELISA and compared with the model control group.One-way analysis of variance(One-Way ANOVA)was used in the group,and LSD method was used between the groups.P <0.05 was considered as a significant difference.Results:1.Establishing GIOP model: After 8 weeks of modeling,comparison of bone biochemical indicators,group A is lower than group B: alkaline phosphatase(ALP)group B is lower than group A,the difference is significant(P<0.05);osteocalcin(BGP)Group B was significantly lower than group A with significant differences(P<0.05);calcium(Ca)and phosphorus(P)were not significantly different between groups A and B(P>0.05);left femur bone density(BMD)CT images showed significant differences between group B and group A(P<0.05).Group B had a larger hollow area and disordered trabecular bone fracture.The left femur bone mineral content(BMC)showed a significant difference between group B and group A.(P<0.05);the microstructure parameters of model control group B were significantly lower than those of blank control group A(Tb.Th)and the number of trabecular bones(Tb.N)(P<0.05);Trabecular separation(Tb.Sp)significantly increased(P<0.05);relative bone volume or bone volume fraction(BV/TV)decreased significantly(P<0.05);BS / BV bone surface area bone volume ratio(BS/BV)Significantly increased(P<0.05);but there was no significant difference in the parameter values between the Chinese medicine treatment group C and the blank control group A(P>0.05).The trabecular bone of C bone in the Chinese medicine treatment group improved,and local repair was better.There were also differences in HE imaging images of the right femur.The bone cavity in group B was obvious and the bone microstructure was damaged.In short,GIOP was successfully modeled by comparing bone biochemical indicators and bone microstructures in the model group and the blank control group.2.Prevention and treatment of Zhuanggu Tablets on GIOP(1)Changes in body weight: At the beginning of the fourth week of the experiment,the weight gain of the four groups of rats began to show a significant difference(F=6.397;P=0.001<0.05).The weight of group A was significantly higher than those of groups B and D(P=0.000,0.007<0.05);there is no significant difference between group A and group C(P=0.056>0.05);the weight of group B is significantly lower than that of groups A and C,and the difference is significant(P=0.000,0.028<0.05);There was no significant difference between groups B and D(P=0.170>0.05);there was no significant difference between groups C and D(P=0.378>0.05).(2)Changes in bone biochemical indicators: ALP and BGP were significantly different between the four groups A,B,C,and D(F=6.153,18.806;P=0.002,0.000<0.05);both group ALP and BGP were Lower than group A with significant difference(P=0.042,0.003<0.05);no significant difference in ALP between groups A and C and D(P=0.225,0.068>0.05);significant difference in BGP between groups A and C and D(P=0.01,0.001<0.05);ALP and BGP in groups C and D were higher than those in group B with significant differences(P=0.002,0.000<0.05,P=0.000,0.000<0.05);See obvious abnormalities(Fcalcium=1.664,Pcalcium=0.192;Fphosphorus = 1.509,P phosphorus = 0.229).(3)Changes in bone mineral density: The left femur bone mineral density was significantly different between the four groups A,B,C,and D(F = 4.736,P = 0.007<0.05);the left femoral bone mineral density of group B was significantly lower than that of group A. Difference(P = 0.002 <0.05);there was no significant difference between group A and C and D(P = 0.506,0.621>0.05);group C and D were higher than group B(P = 0.009,0.006 <0.05);C,There was no significant difference between the two groups(P = 0.864>0.05);the left femur bone mineral content showed that there was a significant difference between the left femur bone mineral content among the four groups A,B,C,and D(F = 18.57,P = 0.000<0.05).There was a significant difference between group B and group A(P = 0.000 <0.05).Groups C and D were higher than group B and there was a significant difference(P = 0.000,0.000 <0.05).There was no significant difference between groups C and D(P = 0.22>0.05);the difference between A and C groups was significant(P = 0.008<0.05),but there was no significant difference between A and D groups(P = 0.56>0.05).(4)Changes in bone-related parameters: BV / TV,BS / BV,Tb.Th,Tb.N,and Tb.Sp were significantly different between the four groups of bones A,B,C,and D(F=7.648,10.449,4.086,4.913,10.533;P = 0.000,0.000,0.014,0.006,0.000<0.05);BV / TV,Tb.Th,and Tb.N of the bone microstructure in group B were lower than those in group A with significant differences(P = 0.004,0.026,0.002<0.05);BS / BV,Tb.Sp is higher than group A with significant differences(P = 0.000,0.000<0.05);BV / TV,Tb.Th,No significant difference was found in Tb.N and Tb.Sp(P = 0.720,0.131;0.922,0.283;0.612,0.975;0.109,0.267>0.05);there was no significant difference in BS / BV between groups A and D(P= 0.103>0.05);BS / BV in groups A and C were significantly different(P = 0.009 <0.05);BV / TV,Tb.Th,Tb.N in groups C and D were higher than those in group B and the differences were significant(P = 0.002,0.000 0.033,0.002;0.009,0.002<0.05);BS / BV and Tb.Sp in groups C and D were lower than those in group B with significant differences(P = 0.010,0.001;0.001,0.000 <0.05).(5)Bone morphological changes: There were significant differences in bone imaging and bone microstructure between group B and the other three groups.There were no significant imaging differences between groups A,C and D.CT imaging of left femoral bone mineral density showed significant differences between group B and group A(P <0.05).Group B had a larger cavity area and trabecular structure disorder was fractured.The microstructure of bone tissue was observed by HE staining.It was found that the width,number,and area percentage of trabeculae in the model group were lower than those in group A,and the trabecular bone separation was greater than that in group A.Conclusion:1.The decrease in bone mineral density and bone mineral content in the rat model group and HE staining indicated that dexamethasone could successfully establish the GIOP model.However,there are individual time differences in the specific modeling time and whether the difference is significant.2.Chinese herbal compound Zhuanggu tablets can prevent the occurrence of GIOP,and its preventive effect and the effect of calcium Erqi D promote each other;the weight of the group with the intervention of traditional Chinese medicine increases faster.By improving bone biochemical indicators,it can inhibit bone resorption and reduce bone mass Loss can prevent osteoporosis.3.Enrich the scientific and rational connotation of the theory of "kidney sperm and essence". |
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