The Effect Of Shugan Huazhi Capsule On Adiponectin And Its Receptor In NAFLD Rats

Purpose:Nonalcoholic fatty liver disease(NAFLD)is a kind of liver disease characterized by abnormal lipid metabolism.At present,there is no specific treatment for the treatment of this disease.This experiment studied the effect of Shuganhuazhi Capsule on adiponectin and its receptors in NAFLD rats,and explored the therapeutic effect of Shuganhuazhi Capsule on NAFLD rats in order to provide experimental basis for clinical drug use.Material and method:Thirty-three male Sprague-Dawley Sprague-Dawley(SDF)healthy SD rats were fed one week later.The weights of the rats were weighed.The rats were randomly divided into three groups according to the weight of the rats: normal group,model group and Chinese medicine group.10 only.The normal group was fed with common feed,the model group and the Chinese medicine group were fed with high-fat diet.The three groups all had normal drinking water.The feeding environment was the same,the quietness was undisturbed,and the temperature and humidity were kept within a suitable range.After observing the oil red O staining,the histopathological changes of the liver were observed.whether succeed.After successful modeling,the Chinese medicine group used the drug to intervene in the process of treatment,the normal group of rats according to body weight according to the standard volume of 1ml/100 g rat body weight perfused with the corresponding volume of physiological saline,sooner or later;the model group continue to give The high-fat diet was fed to maintain the stability of the model.At the same time,according to the weight of the rats in this group,the corresponding volume of physiological saline was fed according to the standard of 1ml/100 g rat body weight.The Chinese medicine group continued to give high fat.Feed feeding,according to the weight of rats in the group according to the standard of the volume of 1ml/100 g rat body weight of the stomach to give the appropriate volume of the drug suspension with a good Shuganhuazhi capsule,once each morning and evening.Gavage time was 4 weeks.After the experiment,the serum biochemical indicators,adiponectin levels,and liver AdipoR2 protein and mRNA expression levels were measured and analyzed in each group.The effects of Shuganhuazhi capsules on adiponectin and its receptors in NAFLD rats were observed.Results: 1.Results of serum biochemical indicators in each group: Compared with the normal group,the levels of serum TG,TC,and LDL-C in the model group were significantly increased(P<0.05),and the HDL-C content was significantly reduced.The difference was statistically significant(P<0.05).The levels of serum TG,TC,and LDL-C in the Chinese medicine group increased slightly,the difference was not statistically significant(P>0.05),the HDL-C content was slightly decreased,and the difference was not statistically significant.Significance(P>0.05).Compared with the model group,serum TG,TC,and LDL-C levels in the traditional Chinese medicine group were significantly lower(P<0.05),and HDL-C levels were significantly higher(P<0.05).2.Liver histopathological changes in each group: The gross observation of the liver showed that the livers in the normal group were normal in size,reddish-brown in color and smooth in the capsule;there was no change in liver shape in the model group and the Chinese medicine group.The capsule is tense,the edge is blunt and thick,the surface is smooth,the liver is yellow,and it has a greasy feel.Observed under the light microscope,the normal group of liver cells without fat deposition,cell morphology and size are not abnormal;both the model group and the Chinese medicine group rats have lipid degeneration,visible most fat particles,fat deposition is obvious.3.The serum ADPN level in each group: Compared with the normal group,ADPN levels in the serum of the model group were significantly lower(P<0.05);serum ADPN levels in the Chinese medicine group were slightly lower.The difference was statistically significant(P<0.05).Compared with the model group,ADPN levels in the serum of the Chinese medicine group were significantly higher,and the difference was statistically significant(P<0.05).4.Results of AdipoR2 mRNA expression in liver of rats in each group:Comparative analysis of liver AdipoR2 mRNA expression levels in three groups of NAFLD rats showed that compared with the normal group,the expression level of AdipoR2 mRNA in the model group was significantly lower.Statistical significance(P<0.05);The Chinese medicine group was slightly lower than normal,and the difference was statistically significant(P<0.05).Compared with the model group,the expression of AdipoR2 mRNA in the TCM group was significantly higher than that in the model group,and the difference was statistically significant(P<0.05).5.Effect of NAFLD rat liver AdipoR2 protein expression:Comparative analysis of the three groups of rat liver AdipoR2 protein gray ratio ratio,found that the three groups of rat liver AdipoR2 protein expression levels are different(P<0.05),with normal The group has the highest expression level.Compared with the normal group,the protein gray ratio of liver AdipoR2 in the model group rats was significantly lower(P<0.05);compared with the normal group,the protein gray ratio of the liver AdipoR2 rats was slightly lower.The difference was statistically significant(P<0.05).Compared with the model group,the protein gray ratio of liver AdipoR2 was significantly higher in the Chinese medicine group than in the model group,and the difference was statistically significant(P<0.05).Conclusion: 1.Shuganhuazhi Capsule can significantly improve liver function(ALT,AST)and blood lipids(TG,TC,HDL-C,and LDL-C)levels in serum of NAFLD rats,and can significantly improve liver fat deposition...2.Shuganhuazhi Capsule can promote the expression of Adipo R2 protein and mRNA in NAFLD rats by increasing serum adiponectin level in NAFLD rats.