| Objective:MicroRNAs(miRNAs)are short(about 22 nucleotides in length)single-stranded non-encoding RNAs that regulate gene expression at the post-transcriptional level,playing key roles in the pathologic process of heart failure.MicroRNAs were recently discovered to circulate in the bloodstream in a remarkably stable form,becoming the focus of biomarker research.Our previous study screened plasma microRNAs which differentially expressed in rats with experimental heart failure.The objective of this research was to explore the effect of Shenfu Decoction on cardiac function and plasma microRNAs expression in rats with experimental heart failure,and find the potential mechanism on microRNAs.Methods:Experimental rats were randomized into sham-operated group,heart failure group,and Shenfu Decoction group.Experimental heart failure was induced by ligation of the left coronary artery anterior descending branch.4 weeks after the surgery,echocardiography was used to evaluate the cardiac function,and the rats with cardiac ejection fraction(EF)<45%were chosen as heart failure models,whieh were randomized into heart failure group and Shenfu Decoction group.sham-operated group and heart failure group rats were given with saline water,while Shenfu Decoction group rats were treat with Shenfu Decoction(0.5g decoction pieces/mL,lml/100g,once a day)for 4 weeks.Echocardiography was used to evaluate the cardiac function,ELISA immunoenzymatic method was used to detect serum levels of N-terminal prohormone brain natriuretic peptide(NT-proBNP),and the right carotid artery was cannulated to record the hemodynamic parameters.The Plasma microRNA expression was detected by quantitative reverse transcription-PCR(qRT-PCR).Results:Compared with the sham-operated group,EF and FS in the ligat ion group were s ignif icant ly lower(P<0.05).At the same t ime,LVIDd,LVIDs,LVEDV and LVESV in the ligation group were significantly higher(P<0.05).Compared with the heart failure group,EF and FS in the Shenfu Decoction group were significantly higher(P<0.05),while LVIDd,LVIDs,LVEDV and LVESV have not significant difference(P>0.05).Compared with the sham-operated group,LVESP,+dp/dtmax,and-dp/dtmax in the heart fai lure group were significantly lower(P<0.05),while LVEDP was significantly higher(P<0.05).Compared with the heart failure group,LVESP,+dp/dtmax,and-dp/dtmax in the Shenfu Decoction group were signif icantly higher(P<0.05),while LVEDP was significantly lower(P<0.05).Compared with the sham-operated group,the serum levels of NT-proBNP in the heart failure group was significantly higher(P<0.05).When compared with the heart failure group,the serum levels of NT-proBNP in the Shenfu Decoction group were significantly lower(P<0.05).Candidate control gene(mir-16 and mir-17-5p)expression in plasma have not significant difference in different groups(P>0.05).Compared with the sham-operated group,plasma mir-122 expression level was significantly higher in heart failure group(P<0.05).When compared with the heart failure group,plasma mir-122 expression level was significantly lower in the Shenfu Decoction group(P<0.05).However,others(mir-423-5p,mir-21,mir-1,mir-7b,and mir-206)expression in plasma have not significant difference in different groups(P>0.05).Conclusion:Shenfu Decoction can significantly improve the cardiac function in rats with experimental heart failure,increase the EF,decrease the serum levels of NT-proBNP,and improve the hemodynamic parameters,which may lead to the treatment effect of Shenfu Decoction on heart failure. |
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