Research On The Screening And Action Mechanism Of Medicaments Based On Plant Virus Coat Protein Targets

In recent years,plant virus diseases have caused huge losses in the quality and quantity of agricultural products in the agricultural production process.Among them,the virus diseases caused by Potato virus Y(PVY)are particularly serious.Although there are some basic advances in the disease,there are a few agents on the market that can effectively treat the disease,but the specific mechanism of action is still unclear,making it difficult to develop specific agents for the pathogen.How to effectively screen anti-PVY drugs is a key issue in the prevention and control of potato virus diseases.Therefore,it is important to establish a drug screening model with PVY key proteins as potential targets and to find highly active anti-PVY drugs,which is of great significance for the prevention and control of potato virus diseases.In this paper,the prokaryotic expression of purified potato virus Y protein(CP)was used as the target,and the PVY-CP and anti-resistance were studied by isothermal calorimetry,micro-thermometry,Agrobacterium-mediated method and Western blotting.The interaction between small virus molecules,the binding constant between PVY-CP and antiviral small molecules,the number of binding sites and the main mode of action were measured.According to the force of the drug and PVY-CP,the anti-PVY drug screening was performed at an ex vivo level.Subsequent two experimental experiments of mutant proteins and living plants initially explored the mechanism of action of the drug,and established a drug screening model based on PVY-CP as a potential target.The results showed that ribavirin,morpholinium hydrochloride and PVY-CP had different degrees of binding in vitro.The binding constant was ribavirin(4.56 ?M)<morpholinium hydrochloride(77.6 ?M).However,amino oligosaccharides and benzothiadiazoles did not bind to PVY-CP,so it was judged that there was no interaction between them;then the interaction between the myricetin derivatives and PVY-CP was studied,and the results showed that LP4,LP6,LP9 and Both LP17 and PVY-CP have different degrees of binding,and the binding ability of LP6 and LP9 to PVY-CP is stronger than that of LP4 and LP17 and PVY-CP.The binding sites of ribavirin and PVY-CP were confirmed by arsenic protein verification experiments as arginine(R)153,arginine(R)179,aspartic acid(D)197 and lysine(K)217.In vivo verification experiments showed that ribavirin inhibited the expression of PVY-CP in living plants.