Font Size: a A A

Agrobacterium-mediated Infection Method Resilience Of Upland Rice APX Gene Conversion

Posted on:2015-01-06Degree:MasterType:Thesis
Country:ChinaCandidate:X Y ZhangFull Text:PDF
GTID:2180330425986948Subject:Botany
Abstract/Summary:PDF Full Text Request
China is a large agricultural country, rice is not only an important food crop in ourcountry, but also one of the important commodity grain, relationship to the nationaleconomy and national food security. Cold damage, drought, high salt production is thethree main factors that cause the decrease of rice production. Currently, foster resiliencethrough new varieties of genetically modified method is an effective way to improve theresistance of rice.When a plant subjects to environmental stress, a large amount of active oxygen willaccumulate in the plant cell, if the ineffective removal of active oxygen will cause damageto plants.Ascorbate peroxidase enzymes is an important enzyme in enzymaticdetoxification system, which is a heme-containing proteins in plants primarily removeH2O2, in order to improve the resilience capabilities of the plant.APX is a key enzyme in removing H2O2, for the protection of chloroplasts and othercellular components from H2O2and Generated hydroxyl radical damage is essential. WhenH2O2accumulation in plants,it can quickly induce the expression of APX, APX ascorbicacid and H2O2catalytic reaction, H2O2is decomposed. APX plays an important role inresistance to oxidative stress in plants.The topics use the callus induced by upland rice1stmature embryo as transformedreceptor.Transform APX genes into rice by Agrobacterium infection method and access tothe resistance significantly enhanced transgenic plants.
Keywords/Search Tags:APX, uplang rice, stress resistance, agrobacterium-mediated transformation
PDF Full Text Request
Related items