High-Throughput Rapid Detection For Biochemical Molecules Based On LEGO And Smart-phone Two-Dimensional Code Decoding Technology

As one of the earliest graphics recognition technologies,bar code technology has been widely used in many fields of life due to its convenient use and high reliability,which has greatly improved people's lives.The widespread popularity of smart phones has also greatly promoted the development of bar code technology,and the concept of smart phone scan code is more deeply rooted in the hearts of the people.As a new molecular biology technology,biochip technology has the problem of difficult to accurately locate samples while achieving high-throughput detection.Biochip technology and bar code technology are combined to prepare barcode-based biochemical chips,and intelligence is utilized.The mobile phone decodes the barcode-type chip,and realizes the high-throughput detection of biochemical molecules,and can also effectively solve the problem of sample localization.The barcode-based biochip provides a new detection method for the detection field.This study proposes a high-throughput detection technique for biochemical molecules based on two-dimensional code patterns.This technology encodes the position of the detection channel in the biochip according to the codec principle of the two-dimensional code,and digitally reads the detection chip of the two-dimensional code pattern by developing a smartphone app on the premise of the color reaction of the biochemical molecule.It can simultaneously realize rapid detection of various biochemical molecules.The specific research contents of this paper are as follows:1.The LEGO modules and the transparent channels of 3D printing are used to splicing the biochemical detection platform of the two-dimensional barcode pattern on the LEGO board,and the transparent channel is used for the color reaction of the biochemical molecule.A smartphone app was developed based on the Java language,which enables digital reading of the 2D barcode platform through a specially designed QR code threshold scanning function.A large database representing the decoding result and the positional relationship is established in the program,and according to the decoding result,accurate positioning of the dark detecting unit in the detection area can be realized.2.Heavy mental ions Cr(VI)and Ni(II)in water can be detected by this system simultaneously.The gray threshold in decoding program is calibrated according to the emission standard of Cr(VI)and Ni(II)in sewage.The result of the decoding is the position of the detection unit corresponding to the concentration of heavy metal ions in the detection zone exceeding the standard value.The test indicators meet the requirements for Cr(VI)and Ni(II)in the comprehensive wastewater discharge standard.The feasibility of the system for the detection of heavy metal ions in tap water was confirmed by the method of tapping the water sample.3.Triglycerides,total cholesterol and uric acid in blood can be achieved by this detection system simultaneously.The threshold value in the mobile phone decoding App is calibrated according to the criteria of the three indicators in healthy human blood.Through the continuous decoding operation,the unit position of the three indicators whose concentration in the detection zone exceeds the standard can be obtained.The system's detection of indicators meets the requirements for triglycerides,total cholesterol and uric acid in human blood health standards.In addition,the detection of the actual blood sample was completed by this detection system,and the reliability of the detection system was confirmed by comparing with the results quantified by the hospital.