| In order to study the effect of high-sugar diet on the distribution density and morphological function of argyrophils in the digestive tract of mice(Rattus norvegicus),this experiment used the Grimelius silver staining method to observe and measure the morphological function of argyrophils in the digestive tract of high-sugar diet for 10 weeks And density distribution.The results showed that in 2 weeks,4weeks,6 weeks,8 weeks,and 10 weeks,the control group(normal feeding group)and the experimental group(the high-sugar diet group)had arginophils in the digestive tract of all mice except the esophagus Distribution of argyrophils.The morphology of the argyrophils in the digestive tract of the two groups of mice was different.The control group was mainly Ellipse and Cone-shaped.The experimental group was mainly Cone-shaped.The peak density of the distribution of argyrophils in the digestive tract of the control group and the experimental group was located in the stomach.The number of argyrophils in the experimental group gradually increased from 2 to 6 weeks,and then decreased to 10 weeks.Compared with the experimental group,the number of arginophils in the gastric cardia,stomach,pylorus,and duodenum of the experimental group was significantly different at the 6th week(p<0.01),the duodenum was significantly higher than the control group(p<0.05),and at the 10 th week of the experiment,the cardia,stomach and pylorus of the experimental group were significantly lower than the control group(p<0.05).The difference in the number of argyrophils in the digestive tract of the experimental group of mice was not significant,indicating that feeding on a high-sugar diet will significantly change the morphology and number of argyrophils in the digestive tract of mice,which may be related to changes in the function of endocrine cells.At the same time,the localization of gastrin cells(Gas)cells and glucagon(Glu)cells in the digestive tract was studied by avidin-biotin complex methods(ABC).The results showed that Gas cells of the control group and the experimental group were distributed in the pylorus、duodenum and jejunum,but there was no distribution in other parts.The Gas cells of the two groups of mice were different in morphology.Thecontrol group was mainly Ellipse and Cone-shaped,The shape of the cells in the experimental group was mainly Cone-shaped.The peaks of the distribution density were located in the pylorus.After being fed with a high-sugar diet,the number of gas cells in the gastrointestinal tract of the experimental group decreased from 2 to 10 weeks.Compared with the control group,the distribution density of gas cells in the gastrointestinal tract of mice in the experimental group at the 10 th week was very different from that in the pylorus,and the number was significantly lower than that in the control group(P <0.01).Between the groups,the difference was not significant.Glu cells in the digestive tract of the control and experimental groups were distributed in the duodenum 、 jejunum 、 ileum 、 colon and rectum.The other parts were not distributed.The morphology of the Glu cells in the digestive tract of the two groups of mice was similar.The control group was mainly Ellipse and Cone-shaped.The experimental group is dominated by Cone-shaped and Round.The peaks of distribution density are located in the ileum.In the second and fourth weeks of the experiment,the number of Glu cells increased significantly.Ileum Glu cells were significantly higher than those in the control group(P <0.01)and rectum were significantly higher than those in the control group(P <0.05).The other parts were not significantly different between the control group and the experimental group.From the 4th week to the 10 th week,the number of Glu cells in the digestive tract of the experimental group of mice decreased again.There was no significant difference in the number of Glu cells between the two groups of mice.This may be related to the physiological functions of different parts of the mouse digestive tract. |
