Sports Injury Of The High Methionine Diet Rat Aortic Endothelial Cells And Its Mechanism

Objective: Observe training effects on aorta endothelial cell of rats supplied with the high methionine diets , inquiry into the feasible aerobic sports prevent from the endothelium hurt resulted with Hcy and its mechanisms, thus exercise provides the new academic bases for the prevention and cure of the hyperhomocysteinemia and the AS occurrence and development.Method: The male Wistar rats are randomly divided into the normal food matched control( C ), high methionine diets group ( M ) and sports+ hypermethionine diets group( S). S and M group are feed with high methionine feedstuff containing 3% methionine, and S carries on 90 minutes swimming. C feeds normal feedstuff; all rats are totally raised for 8 weeks.The plasma Hcy, plasma MDA content, vitality of SOD and content of GSH, the NO/ ET balances, the TXB₂/6- Keto- The PGF_1α balances and the t-PA/ PAI balances are used to evaluate endothelium function and use scanning electron microscopy to observe endothelium form. Result: Adopt 11.0 software packages of SPSS to these results:1. Compared with C , M group plasma Hcy content goes up obviously ( P<0.01) ; T group plasma Hcy slightly goes up, but the difference show unobvious ( P>0.05) ; Compared with M, T group plasma Hcy obviously descends , it shows that the feat sport can lower the hypermethionine diets rats plasma Hcy level, preventing from the formation of the hyperhomoeysteinemia.2. Compared with the C, M group plasma MDA content and SOD goes obviously up (P<0.05), it shows that the hypermethionine diet can induce oxidation stress.Compared with the M, T group GSH content obviously increase (P<0.05), and T group plasma SOD vitality and GSH content obviously increase compared with the C (P<0.05);it shows that the feat exercise can enhance the ability of oxidation resistance, polish up oxidation stress inside and prevent endothelium injury aroused by Hcy.3. Compare with C group: The plasma NOS vitality of M group descended, the NO content obviously descended, the plasma ET obviously increased, the NO/ET decreased ( P<0.05), it shows that the hypermethionine diet rats plasma NO/ ET be out of balance, expressing the obstacle of blood vessel tension regulation. Compare with M group: plasma NOS vitality, the NO content and NO/ET balance of T groupobviously ascend, plasma ET descended without difference(P>0.05). Compared with C, all the indexes of T group are no difference (P>0.05). Explaining that the feat sport can improve the hypemethionine diet rats plasma NO system and a balance between NO and ET obviously, prevent blood vessel tension from regulation obstacle with the result that AS occurrence and development.4. Compare with C group: The plasma 6-Keto-PGF_1αof group M descended obviously, plasma TXB₂ content obviously ascended, the plasma 6-Keto-PGF_1α/TXB₂ decreased ( P<0.05), it shows that the hypermethionine diet rats plasma6-Keto-PGF_1α-TXB₂ be out of balance, expressing the blood vessel convulsion and blood platelet congregates intensely. Compare with M group: plasma TXB₂ content of T group obviously ascend, plasma 6-Keto-PGF_1α and 6-Keto-PGF_1α/TXB₂ descended obviously (P<0.01), Compared with C, all the indexes of T group are no difference (P>0.05). Explaining that the feat sport can improve the hypemethionine diet rats plasma balance between TXB₂ and 6-Keto-PGF_1αobviously on resisting blood platelet congregation and the blood vessel convulsion, preventing AS and thrombus complications.5. Compare with C group: The plasma t-PA content of M group descended obviously, plasma PAI content obviously ascended, the plasma t-PA/PAI decreased(P<0.05), it shows that the hypermethionine diet rats plasma t-PA/PAI be out of balance, expressing fibrinolytic function foul-up. Compare with M group: plasma t-PA content of T group obviously ascend, plasma PAI and t-PA/PAI descended obviously (P<0.01); Compared with C, all the indexes of T group are no difference (P>0.05). Explaining that the feat sport can improve the hyper...