The Regulation Of AMPK And Akt On The Synthesis Of Hepatic Glycogen In Rats During Different Phases After Exhaustive Exercise

Objective: The rats were subjected to a one-time exhaustive load test to observe the recovery of glycogen in the liver and some indicators which reflect glycogen synthesis during different phases of recovery.Then,we explored the application of carbohydrate supplementation in exercise.Method: 40 SD rats were randomly divided into 4 groups.All rats in the exercise groups were subjected to an increased load exhaustion experiment,and were sacrificed at 0h,6 h and 24 h after exercise,and changes of the indicators in liver were measured.Statistical data were processed with one-way ANOVA.Result:(1)The contents of hepatic glycogen were lower in each exercise group than what in the quiet group,and the content of hepatic glycogen in the 24 h group was significantly lower than that of the quiet group(p<0.01)and the 0 h group(p <0.01).(2)The activities of GS decreased gradually with the recovery after exercise.GS's activities in the 24 h group were significantly lower than those in the quiet group(p <0.05).(3)The activities of GSK increased gradually with the recovery after exercise.GSK's activities in the 24 h group were significantly higher than those in the quiet group(p <0.05).(4)The phosphorylation level of AMPK in the 0H group was significantly lower than that of the quiet group(p <0.05),and there was no significant difference between the other groups.(5)The level of AKT phosphorylation at 12 h after exercise and 0h after exercise was lower than that of quiet group,and it was higher at 24 h after exercise than that of quiet group,but there was no significant difference.Conclusion:1.After the exhaustive exercise,the liver glycogen synthase activity gradually decreased within 24 hours,which resulted in the inhibition of glycogen synthesis rate.2.The level of phosphorylated AMPK in the rats decreased significantly after exercise,and then gradually increased,but there was no significant change in the expression of Akt protein.Under the influence of these,the level of hepatic glycogen synthase kinase 3 was increased,which in turn produced an effect of enhancing the activity of inhibiting glycogen synthase.