Study On The Characteristics Of Intestinal Microbiota In Patients With Chronic Atrophic Gastritis With Thin White Fur And Yellow Greasy Fur

Objective: To study the intestinal microbiota characteristics of representative tongue fur(thin white fur and yellow greasy fur)in patients with chronic atrophic gastritis(CAG),and to analyze and compare the distribution differences of intestinal microbiota between them.The purpose of this study is to investigate the relationship between different tongue fur and intestinal microbiota in patients with CAG,and to explore the possible mechanism of the formation of tongue fur in CAG patients from the point of view of intestinal microbiota,so as to provide a basis for preliminary research on whether traditional Chinese medicine can improve the degree of illness and the state of tongue fur by adjusting intestinal microbiota.Methods: 31 CAG patients with thin white moss,30 patients with yellowish greasy moss and 30 healthy controls were selected from the Department of spleen and stomach Diseases,Longhua Hospital affiliated to Shanghai University of traditional Chinese Medicine.The basic information,clinical symptoms,gastroscopic manifestations and pathological reports of CAG patients were collected.The pictures of tongue fur of CAG patients and healthy controls were taken under natural light,and fecal samples were collected.The diversity and abundance of intestinal microbiota in patients with CAG were studied by 16 Sr DNA gene sequencing and bioinformatics.Results: 1.There was no significant difference in sex,age and BMI between CAG thin white fur group,CAG yellow greasy fur group and healthy control group(P>0.05).There was no significant difference in symptom score,pathological score(chronic inflammation,activity,atrophy,intestinal metaplasia)and Hp infection between CAG thin white fur group and CAG yellow greasy fur group(P>0.05).2.There was no significant difference in the richness,evenness and diversity of intestinal microbiota between CAG patients and healthy controls(P > 0.05).3.The richness of intestinal microbiota in CAG patients with yellow greasy fur was higher than that in CAG patients with thin white fur(ace,chao index P < 0.05).4.According to the significance test of the difference between groups,at the phylum level,the abundance of Firmicutes and Actinobacteria in fecal of CAG patients was lower than those in fecal of healthy controls,while the abundance of Bacteroides and Saccharibacteria in fecal of CAG patients was higher than that in fecal of healthy controls(P < 0.05).At the genus level,the abundance of Bifidobacterium,Dorea,Fusicatenibacter and [Eubacterium]?hallii?group in fecal of CAG patients was lower than that in healthy controls,while the abundance of Bacteroides in CAG patients was higher than that in healthy controls(P < 0.05).5.According to the significance test of the difference between groups,at the phylum level,the abundance of Fusobacteria and Saccharibacteria in fecal of CAG patients with yellow greasy fur were less than that in CAG patients with thin white fur(P < 0.05).At the genus level,the abundance of Subdoligranulum in fecal of CAG patients with yellow greasy fur was higher than that in CAG patients with thin white fur(P < 0.05).6.According to LEf Se analysis,from gate level to generic level,the abundance of 11 species such as g?Dorea,g__Fusicatenibacter and f__Coriobacteriaceae in the healthy control group increased significantly.The abundance of 9 species such as f__Lactobacillaceae,c__norank?p__Saccharibacteria and o__Campylobacterales in the CAG thin white greasy group increased significantly.The abundance of 26 species such as c__Bacteroidia,g__Prevotella?7 and o__Rickettsiales in the CAG yellow greasy fur group increased significantly(P < 0.05).Conclusions: 1.Between CAG patients and healthy controls,there were differences in the composition of intestinal microbiota from gate level to generic level.2.The richness of intestinal microbiota in CAG patients with yellow greasy fur was significantly higher than that in patients with thin white fur.3.There was a statistically significant difference in the composition of intestinal microbiota between CAG patients with yellow greasy fur and those with thin white fur,which may affect the formation of tongue fur in CAG patients.