Characteristics Of Gut Microbiota In Patients With Ulcerative Colitis With Thin White Fur And Yellow Greasy Fur

Objective:The purpose of this study was to observe the characteristics of gut microbiota in patients with ulcerative colitis?UC?and healthy persons,UC patients with thin white fur?UC with TWF?and yellow greasy fur?UC with YGF?,and to analyze the distribution difference of between UC with TWF and UC with YGF.To explore the formation mechanism of tongue coating in patients with UC,and to lay the experimental foundation for further study of the mechanism of tongue coating formation in UC patients.Methods:Thirty-five patients with UC thin white fur,35 patients with UC yellow greasy fur and 35 healthy persons?thin white fur?who met the inclusion criteria were selected and fecal specimens were collected.The diversity and abundance of intestinal flora in UC patients were studied by 16S rDNA gene sequencing and bioinformatics.Results:1.There was no significant difference in age,surname and BMI in UC with TWF group and UC with YGF group and HCs group?P>0 05?.There was no significant difference between UC with TWF group and UC with YGF group in the course of disease,range of pathological changes,disease activity,score under enteroscopy and take medicine?P>0 05??2.Compared with the HCs group,the species richness and diversity of the gut microbiota in UC with TWF group and UC with YGF group decreased significantly?P<0.001?.The abundance and diversity of the gut microbiota of UC with YGF group were lower than those of UC with TWF group,but there was no statistical difference?P>0.05?.3.phylum level1)Compared with healthy people,the gut microbiota of UC patients was significantly reduced in Firmicutes,Verrucomicrobia,Tenericumes,and Chloroflexi?P<0.05?,Saccharibacteria increased significantly?P<0.05?.In addition,Bacteroidetes,Actinobacteria,and Proteobacteria in the gut microbiota of UC patients showed an increasing trend,but there was no statistical difference?P>0.05?.2)Compared with the HCs group,Firmimutes,Verrucomicrobia,Tenericutes,and Chloroflexi were significantly reduced in the gut microbiota of the UC with TWF group?P<0.05?.3)Compared with the HCs group,Firmimutes,Verrucomicrobia,Synergistetes,and Chloroflexi were significantly reduced in the gut microbiota of the UC with YGF group?P<0.05?.4)Compared with the UC with TWF group,Cyanobacteria in the gut microbiota of the UC with YGF group increased significantly?P<0.05?.4.Genus level1)Compared with healthy people,the gut microbiota of UC patients was significantly increased in Escherichia-Shigella,[Ruminococcus]gnavus group and Lactobacillus.?P<0.05?.[Eubacterium]_rectale_group,Dorea,Subdoligranulum,Ruminococcus₂,[Ruminococcus]_torques_group,Fusicatenibacter,Phascolarctobacterium,Roseburia and[Eubacterium]_coprostanoligenes group decreased significantly?P<0.05?.In addition,Bacteroides,Bifidobacterium,and Streptococcus in the gut microbiota of UC patients showed an increasing trend,but there was no significant difference between the two groups?P>0 05?.2)Compared with the HCs group,Escherichia-Shigella,[Ruminococcus]_gnavus_group,Streptococcus and[Ruminococcus]_torques_group were significantly increased in the gut microbiota of the UC with TWF group?P<0.05?.[Eubacterium]_rectale group,Subdoligranulum,Dorea,Ruminococcus 2,and Roseburia decreased significantly?P<0.05??3)Compared with the HCs group,Escherichia-Shigella,[Ruminococcus]_gnavus_group,and Lactobacillus were significantly increased in the gut microbiota of the UC with YGF group?P<0.05?.[Eubacterium]_rectale_group,Dorea,Subdoligranulum,Ruminococcus 2,Fusicatenibacter,[Ruminococcus]_torques_gr oup,Phascolarctobacterium and Roseburia decreased significantly?P<0.05??4)Compared with the UC with TWF Streptococcus and Romboutsia in the gut microbiota of the UC with YGF group increased significantly?P<0.05?.5.Analysis of gut microbiota from phylum to genus level1)Compared with healthy people,the gut microbiota of UC patients was significantly decreased in?P<0.05,LDA>2.0?:c_Clostrudia,o_Clostridiales,p_Firmicutes,f_Ruminococcaceae,Eubacterium_coprostanoligenes_group,g_Ruminococcus₂,g_Subdoligranulum,f_Prevotellaceae,g_Alistipes,f_Rikenellaceae,g Fusicatenibacte,g_[Eubacterium]_rectale_group,g_Dorea,f_Porphyromonadaceae,g_[Ruminococcus]_torques group,g Parabacteroides,f_Coriobacteriaceae,o_Coriobacte riales,g_Verrucomicrobiae,g_Verrucomicrobiae,g_Akkermansia and so on.The significant increase in intestinal microflora was found in UC patients?P<0.05,LDA>2.0?:g_Escherichia-shigella and g_[Ruminococcus]_gnavus group,g Lactobacillus,g Erysipelatoclostridium,f_Enterococcaceae,g_Enterococcus,g_Eubac terium_ventriosum_group,g_Anaerotruncus,g_Scardovia,g_Clostridium_innocuum_gro up2,g_Ruminiclostridium₉,Capnocytophaga,p_Saccharibacteria and so on.2)Compared with the UC with TWF group,the gut microbiota of UC with TWF group was significantly decreased in?P<0.05,LDA>2.0?:f_Prevotellaceae,g_Megasphaera,f_Neisseriaceae,f_Prevotellaceae,g_Megasphaera,f_Neisseriaceae,f_Prevotellaceae,g_Megasphaera,f_Neisseriaceae,f_Prevotellaceae,g_Megasphaera,f_Neisseriaceae,O_Neisseriales,g_Neisseria,g_Prevotella 2,g_Alloprevotella,g_Porphyromonas,g_Leptotrichia and so on.The significant increase in intestinal microflora was found in UC with YGF group?P<0.05,LDA>2.0?:c_Bacilli,o_Lactobacillales,f_Streptococcaceae,g_Streptococcus,f_Peptostreptococcaceae,g_Romboutsia?g_Solobacterium and so on.Conclusions:1.The gut microbiota richness and diversity of UC patients were significantly lower than those of healthy people,which showed that the protective bacteria decreased,and the pathogenic bacteria involved in the inflammatory reaction increased,and this performance was more obvious in the UC with YGF group.2.There were differences in gut microbiota composition and bacterial abundance between UC with YGF group and UC with TWF group,which may affected the formation of tongue coating in UC patients.