Mechanism Research Based On Proteomics Analysis Of Specific Changes In Acupoint Blood Flow Perfusion In COPD Rats

ObjectiveTo explore the mechanism of the specific increase of microcirculation blood perfusion of BL13 in rats under the condition of chronic obstructive pulmonary disease(COPD).MethodsHealthy adult male Wistar rats were selected and randomly divided into a COPD model group(C group)and a normal control group(N group).The modeling time was 29 days.Rats of the C group were made model of chronic obstructive pulmonary disease by using passive smoking in the self-made smoking boxes combined with trachea administration of lipopolysaccharide.The N group rats were fed normally during the modeling period,and no treatment intervention was given to both groups.When beginning and finish the modeling,the perfusion unit of all rats' BL13 and non-acupoint beside BL13 were monitored in real time by laser speckle contrast imaging technique.After the establishment of the model,the skin of the two groups of rats' BL13 and non-acupoint beside BL13 were taken to extract the total protein.The proteomics was detected by the tandem mass tag method,and the differential expression protein was analyzed by GO,KEGG,Clustering and Complex Network.Results1.Model replication: The COPD model of rats was successfully reproduced by the improved method.2.Perfusion unit: There was no statistically significant difference in blood flow between all the monitoring points in the two groups before modeling,and BL13 in each group compared with non-acupoint beside BL13(P>0.05).After modeling,perfusion unit of BL13 on both sides of the C group were higher than before modeling(P<0.01),and were significantly higher than those of the non-acupoint beside BL13(P<0.01)and BL13 of the N group(P<0.01).There was no significant difference in perfusion unit of BL13 on both sides of the N group compared with that before modeling(P>0.05),and the right BL13 compared with right non-acupoint beside BL13 were also the same(P>0.05).3.Under the condition of COPD,the differentially expressed proteins detected in CF vs.CP group and CF vs.NF group were enriched in HIF-1 signaling pathway,glycolysis / gluconeogenesis,glucagon signaling pathway and calcium signaling pathway,while the axion guidance was the specific enriched signaling pathway in CF vs.NF group.4.In CF vs.CP group,differentially expressed proteins are mainly involved in the biological process of muscle system process and muscle contraction,and their molecular functions are mainly concentrated in cytoskeletal protein binding,transferase activity,structural molecule activity,and cellular components are enriched in supramolecular fiber.In CF vs NF group,the differential expression proteins are mainly involved in system process and muscle system process,and their molecular functions are mainly concentrated in cytoskeletal protein binding,structural molecule activity and actin binding.Cellular components are enriched in supramolecular fiber and supramolecular polymer.5.The differentially expressed proteins of CF vs.CP group was mainly expressed in prostate and heart,while that of CF vs.NF group was mainly expressed in prostate and esophagus.6.There were significant differences in Tnnc2,Atp2a1,Pgam2,Ryr1,Pygm,Eno3,Gapdh,Aldoa,Pfkm,Ldha,Ppp3 cb,Camk2b,Pgm1,Phka1,Mtor,Phkg1,Phkb,Slc25a4 and Acacb in CF vs.CP group and CF vs.NF group,while Tnnc1,Fbp2,Pgk1,Aldoc and Nfkb1 in CF vs CP group,Cfl2,Ptk2,Hmox1,Rac2,L1 cam,Gnai1,Aldh3a1,Adh7,Plxna4 a,Eif4e2 and Sema3 f were the difference proteins of CF vs.NF group.Conclusion1.In COPD rats,the microcirculatory blood perfusion of BL13 point area showed a specific increase.2.In the state of COPD,the specific increase in blood perfusion in the BL13 acupoint area is mainly related to the activation of HIF-1 signaling pathway,glycolysis / gluconeogenesis,glucagon signaling pathway and calcium signaling pathway.