Influence Of Different Dehydration Incentives On The Support Structure Of Living Materials

Every living body lives in an intricate atmospheric environment.Cells are both the basic constituent unit of each living body and the basic unit that bears the changes of various external environments.It can constitute various organs and tissues upward,and contains many Molecules and atoms,so the meaning of cells to every living body is selfevident.As one of the basic structures to maintain the morphology of life,the cytoskeleton plays a vital role in cells.High pressure,low temperature and osmotic pressure can cause dramatic changes inside the cell,which affects a series of cell physiological functions and can cause changes in plant growth trends.However,it is still unclear how the cytoskeleton regulates its own morphological and structural changes under different dehydration stimulations.This paper further studies the survival conditions of living materials by studying the effects of different dehydration excitations on the supporting structure of living materials.In this project,the cytoskeletal slices can be easily observed by determining the extraction,fixation and washing time of staining reagents.Then,the prepared whole sections were placed under different dehydration excitations(vacuum degree,cooling rate and osmotic pressure)to observe the morphology of the cytoskeleton and the entire cell using an optical fluorescence microscope.The observed cell pictures are stored in the computer,and finally the digital image processing method is used to quantitatively analyze the cytoskeletal morphology pictures under various working conditions,so as to explore the changes of life materials under different dehydration excitations.The paper concludes as follows:1.The continuous vacuum process will cause the cytoskeleton to disaggregate in order to adapt to the dramatic changes in the outside world.This process will gradually increase the value of the cytoskeletal grayscale kurtosis and eventually tend to a relatively stable value.The final relative rate of change was 32.7%.2.The continuous low temperature environment will make the network structure of the cytoskeleton become more sparse until the existence of the skeleton structure cannot be seen in the entire cell.After quantitative analysis through digital image processing,it was found that the cytoskeleton will depolymerize when it is below 0 ?,and the depolymerization phenomenon peaks between-10 and-15 ? and gradually stabilizes.The final relative change rate was 59%.3.The greater the vacuum,the greater the cell deformation.The smaller the cooling rate,the greater the cell deformation.4.The infiltration process will gradually cause the free water in the cell to flow out of the cell.This process will shrink the cytoskeleton and cause disaggregation,thus greatly reducing its mechanical strength;on the other hand,this process will gradually reduce the water content of the cell And gradually increase the pressure in the cell;and too long penetration time(180min is a dehydration time limit)will make the skeleton unable to bear continuous mechanical behavior and structural changes and cause cell inactivation.