Distribution Of Nasal Flora In Chronic Rhinosinusitis By 16SrRNA High-throughput Sequencing

Objective:Through 16 s rRNA high-throughput sequencing of nasal lavage fluid,the nasal flora of chronic rhinosinusitis was investigated and analyzed,and the characteristics of nasal cavity microbiology between different clinical phenotypes were compared,which provided reference information for the study of the microbiological of chronic sinusitis.Method:From September to November 2019,36 patients with chronic rhinosinusitis(CRS)underwent nasal endoscopic surgery in the Second Affiliated Hospital of Nanchang University,including 21 patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and 15 patients with chronic rhinosinusitis without nasal polyps(CRSsNP);According to the infiltration of eosinophils(EOS)in tissues,The chronic rhinosinusitis patients was divided into 12 patients with eosinophil chronic rhinosinusitis(ECRS)and 24 patients with non eosinophil chronic rhinosinusitis(NonECRS).14 patients with nasal septum deviation(control group)were enrolled in the project.nasal lavage fluid was collected and the 16 s rrna v3-v4 variable region of all bacteria in the sample was sequenced using illumina high-throughput sequencing technology(illumina miseq sequencing platform).The sequencing results were clustered in the Operational Taxonomic Unit(OTU)and analyzed using bioinformatics analysis(Alpha diversity analysis,beta diversity analysis,LEfSe analysis)and statistical processing to analyze CRS group and control group(non CRS)and different phenotype CRS nose Characteristics and Differences of nasal flora.Results:The results of sequencing analysis showed that the nasal cavity of CRS and non-CRS patients had abundant and diverse bacterial groups.Bioinformatics analysis showed that at the level of the door were Proteus,Bacteroides,Thick-walled,Actinomycetes,and Clostridia;at the department level,At the genus level of the top five were Corynebacterium,Bacteroides,Neisseria,Haemophilus,Digestion Streptococcus.Compared with non-CRS subjects,the alpha diversity of nasal flora in CRS patients was increased,but there was no statistical difference in the indicators of alpha diversity(chao 1 index,observed index,PD index,and Shannon index)(P> 0.05);Weighted and unweighted PCoA analysis showed no significant difference in ? diversity between the two groups the difference(P> 0.05);the relative abundance of Fibrobacteres was greater in the nasal flora of the CRS group,and the relative abundances ofEggerthellaceae,Fibrobacter,Peptostreptoc-occaceae,Propionibacteriales,Acidobacteriia,and Nocardioidaceae were greater.The relative abundance of Staphylococcaceae and Ambiguous in the nasal flora of the control group(non-CRS)was greater.There was no significant difference in alpha diversity between the CRSwNP group and the CRSsNP group;weighted and unweighted PCoA analysis showed no significant difference in ? diversity between the two groups(P> 0.05);nasal flora Moraxella,Helicobacteraceae,Idiomarina,Acinetobacter,Burkholderiaceae Lactobacillaceae,Vibrionales,Eubacterium,Roseburia,Anaerostipes are relatively more abundant.The relative abundance of Thermoactinomycetaceae in CRSsNP patients was higher.Compared with the NonECRS group,the alpha diversity increased significantly in the ECRS group,and there is a statistical difference in the value of the Shannon index(P = 0.041 <0.05).Weighted and unweighted PCoA analysis showed no significant difference in ?diversity between the two groups The difference(P> 0.05);the nasal flora of patients in the ECRS group was mainly concentrated in the Firmicutes-Lachnospiraceae and Firmicutes-Clostridaceae,Clostridia,Muribaculaceae,Helicobacteracedia,and Ericococeraetriae Abundance is higher.The relative abundance of uncultured_rumen_bacterium in the nasal cavity of patients in the NonECRS group was higher.Conclusion:There are abundant and diverse bacterial groups in the nasal cavity;Differentclinical phenotypes CRS showing different microbial communities.