Developing A Mouse Model Of Acute Bacterial Rhinosinusitis

ObjectiveThe purpose of the study was to develop a mouse model of acute bacterial rhinosinusitis.Subject179 male BALB/c mice. Three dead animals were not counted in the studyMethodA sponge was inserted into the right-side nasal cavities of BALB/C mice in group A, B. In group A, the sponge was impregnated with methicillin resistant Staphylococcus aureus (MRSA COL). In group B, sponge was impregnated with sterile saline. Mice in group C were inoculated with MRSA COL suspension only. Group D was a control group. At day 1,4,7 and 14, the animals were sacrificed. The noses were prepared for microbiological and histological investigation.ResultsAcute bacterial rhinosinusitis was performed in all mice of group A. The inflammation, represented by the number of neutrophils per square millimeter of the mucosa and the percentage of the nasal and sinonasal cavity occupied by neutrophil clusters, was statistically more severe than that in group B. No inflammatory evidence was detected in group C and D.ConclusionAcute bacterial rhinosinusitis mouse model was successfully developed.