| Objective:The metabolic regularity of polymer nanomicelles in kidney tumor specimens from patients with metabolic-related factors was analyzed,and the effect of tumor microvessels on the enrichment of polymer nanomicelle in tumor specimens was initially studied using immunohistochemical microvascular assay technology.To provide a theoretical basis for future targeted treatment of related diseases by polymer nanomicelle drug-loading system.Method:1.Collect clinical data of patients who intending to perform simple polymer nanomicelle perfusion experiments on human isolated kidney tumor specimens by our team,according to the diagnostic criteria:(1)hyperglycemia:FPG?6.1mmol/L(110mg/dl)and/or those who have been diagnosed with diabetes and received treatment;(2)Overweight and/or obesity:BMI?25.0(kg/m~2);(3)Hypertension:systolic/diastolic blood pressure?140/90mmHg and/or those who have been diagnosed with hypertension and treated.The specimens are divided into A:control group:tumor specimens of renal cancer patients without history of hyperglycemia,obesity,hypertension and long-term(lasting for more than 3 months)use of drugs,B:hyperglycemia group,C:obesity group,D:hypertension group,and groups B,C,and D are Single disease group;2.Cyclic perfusion experiment of isolated high molecular nanometer micelles containing protonium in isolated kidney cancer specimens,Scanning tumor specimens using the GSI mode of GE Gem CT,and measuring the enrichment of nanomicelles in the tumor area using the GE ADW4.5 platform.3.The immune tissue SP method was used to detect the expression of CD34 in kidney tumor specimens and the MVD was measured.4.The spss21.0 software was used to statistically analyze the experimental data.Result:1.Twenty-four human kidney tumor specimens were included in this study,including 6 cases in group A,5 cases in group B,7 cases in group C,and 6 cases in group D.The iodine enrichment in group A,B,C,and D all increased with time,and all peaked in about 3-4 hours.The peak values were(3.02±1.07)×100ug/cm~3,(3.99±1.46)x100ug/cm~3,(4.44±0.94)x100ug/cm~3,(2.34±0.52)x100ug/cm~3,the iodine content of group C increased faster and the enrichment was higher after infusion;the microvessel density of group A,B,C,and D specimens were(58.5±2.07)/HP,(62.8±4.15)/HP,(63.6±4.04)/HP,(57.8±5.08)/HP,the microvessel density in group C was higher.2.Compared with the control group,the iodine enrichment and microvessel density in group C were higher than those in group A,which was statistically significant(P<0.05).3.Comparison between different metabolic disease groups,the iodine enrichment and microvessel density in group C were greater than those in group D,which was statistically significant(P<0.05);the iodine enrichment in group B was greater than that in group D,which was statistically significant(P<0.05),but the microvessel density is not statistically significant(P>0.05),suggesting that the density and permeability of tumor vessels in patients with different metabolic diseases are different,leading to different enrichment of iodine.Conclusion:1.The microvessel density of kidney tumors in obese patients is higher,which makes polymer nanomicelles accumulate faster and the amount of enrichment is larger,and its EPR effect is stronger than that of patients without combined metabolic-related factors.2.The microvessel density and permeability of kidney tumors in patients with different metabolic diseases are different,which results in different enrichment and dynamic evolution of polymer nanomicelles,and their EPR effects are different.Obese and hyperglycemic patients have larger nanomicelle enrichment in renal tumor area,and their EPR effect is stronger than that of hypertensive patients. |
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