Expressions Of HIF-1ɑ,MMP-9and MVD In Renal Carcinoma And Its Clinical Significances

Objective To detect the expression of Hypoxia Inducing Factor-1ɑ,MatrixMetalloproteinase-9and Microvascular Density in kidney cancer tissue and adjacentnormal kiney tissue in order to analyze their respective relationship with clinicalpathological parameters and the interrelationship among them.Thus to explore theireffect and clinical significance in the process of infiltration and metastasis of renalcell carcinoma.Method Selection of60kidney cancer specimens as experimental group and20randomly selected tumor-adjacent normal kidney tissue specimens as controlgroup.According to2009AJCC kidney cancer TNM stage standard,these cases aredevided into: Stage Ⅰ19cases, Stage Ⅱ16cases,StageⅢ18cases,Stage Ⅳ7cases.According to the Fuhrman classification:Grade Ⅰ+GradeⅡ36cases,Grade Ⅲ15cases, Grade Ⅳ9cases.Lymph node metastasis in12cases, venous tumor thrombus in22cases. All theses cases have the exclusion of other diseases such as renaltuberculosis, kidney stones, renal infectious diseases, renal benign tumors, renalpelvis tumors, renal mesenchymal tumors, retroperitoneal tumors, etc; and all patientsdidn’t have any radiotherapy, chemotherapy, immunotherapy or targeted therapybefore operation. Using immunohistochemistry (S-P method) to detect theexpression of HIF-1ɑ and MMP-9and MVD in renal cell carcinoma andtumor-adjacent normal renal tissues.Drawing table; Mantel-Haenszel chi-square,ORvalue, t value and Spearman rank correlation analysis were done by using SPSS17.0software.Their respective relationship with clinical pathological parameters and theinterrelationship among them were analyzed.Result The expression rate of HIF-1ɑ in kidneycancer tissue and adjacent normalkidney tissue were58.33%and0%; the expression rate of MMP-9in kidneycancer tissue and adjacent normal kidney tissue63.33%and20%; mean MVDin kidney cancer tissue and adjacent normal kidney tissue were37.93±11.94mm/HP, and22.30±7.89mm/HP, the differences in expression between cancer tissue andnormal tissue were all statistically significant (P <0.05).The expression of HIF-1ɑ,MMP-9and MVD are closely related to clinical stage, pathological classificationand lymph node metastasis (P <0.05, OR value95%confidence interval does notcontain1; there is statistical significance); The expression of HIF-1ɑ, MMP-9andMVD are not related to age, sex, tumor size (P <0.05, OR value95%cicontains1; no statistical significance);in35positive cases of HIF-1ɑ, theMVD value was42.32±10.08,in25negative cases of HIF-1ɑ, the MVDvalue was33.20±12.77, the MVD value of positive cases was significantlyhigher than the MVD value of negative cases(t=3.066, P <0.05); in38positive cases of MMP-9, the MVD value was41.41±10.31, in22negative cases of MMP-9, the MVD value was32.38±13.18, theMVD value of positive cases was significantly higher than the MVD value ofnegative cases (t=2.932, P <0.05); this shows that HIF-1ɑ and MMP-9positiveexpression and MVD are positively correlated. HIF-1ɑ and MMP-9are allpositive in34cases, all negative in20cases, showing a significant positivecorrelation (P <0.05).Conclusion In RCC, HIF-1ɑ, MMP-9and MVD high expression have closerelationship with tumor progression and can be used as important indexes to judge thebiological behavior and forecast the trendency of metastasis. The expression of HIF-1ɑ, MMP-9and MVD are related to clinical stage, pathological classificationand lymph node metastasis,but not related to age and sex,so they may haveimportant effects in the occurrence, development, invasion and metastasis of renal cellcarcinoma. The joint detection of these3parameters may be used as importantindexes for clinical judgment of renal cell carcinoma prognosis, metastasis and otherbiological behavior.