| Objective:To investigate the expression of RTK,CD34 and CD31 in subtypes of RCC and to explore the correlation between the expressions of VEGFR,PDGFR,C-kit,MVD and clinic pathological factors.Methods:CD31 and CD34 were detected in 149 patients with RCC using SP immuno-histochemical technique. The expressions of VEGFR,PDGFR,C-kit were evaluated in 270 patients with different types of RCC using tissue microarray and SP immunohistochemical technique.Results:1. The expression of CD31 and CD34 in clear cell renal cell carcinoma (CCRCC) (98.35±55.05,128.04±46.44) were significantly higher than those in chromophobe renal cell carcinoma (ChRCC) (30.70±17.72,48.55±14.09) and papillary renal cell carcinoma (PRCC) (21.60±9.38,38.12±10.98) (P<0.01).2. The MVD value marked by CD31 (30.70±17.72,21.60±9.38) was much lower than that marked by CD34 (48.55±14.09,38.12±10.98) between ChRCC and PRCC(P<0.01).3. The MVD values marked by CD31 and CD34 were negatively correlated with the pathological grades(rCD34=-0.618; rCD31=-0.442, P<0.01) and clinical stages (rCD34=-0.283; rCD31=-0.256,P<0.05)in CCRCC. But no association was found in non-CCRCC(P>0.05).4. Increased VEGFR-2 expression were significant correlated with tumor size (P=0.016), histological grade(P=0.034) and distance metastasis (P=0.002). VEGFR-3 expression was correlated with histological grade (P=0.028), lymph node status(P=0.010)and distance metastasis (P=0.018).VEGFR-1 expression had no correlation with any clinic and pathologic factors.The expression of VEGFR-2 and VEGFR-3 in died cases were higher than in the alive patients.5. PDGFR-a expression was correlated with histological grade(P=0.001) and distance metastasis(P=0.004). PDGFR-βexpression was correlated with lymph node status (P=0.031) and distance metastasis(P=0.016). PCNA expression was correlated with histological grade(P=0.011). The expression of PDGFR-a and PDGFR-βin died group were higher than the lived group.6. The positive expression rate of C-kit in ChRCC was 94.12%(48/51), it was statistically higher than that in CCRCC(16.06%,22/137)and PRCC (28.05, 23/82)(P=0.001).In ChRCC, the positive expression of C-kit was related with TNM stages. The positive expression of PCNA was related with grade in CCRCC and PRCC. It also had relationship with metastasis in CCRCC.Conclusions:1. MVD was significantly correlated with different type of endothelial labeling. The endothelial cell of microvessels could be shown clearly by its related antigen labeling such as CD34 and CD31. CD34 was more sensitive than that CD31. It could protrusive show smaller and immatured microvessels and even single endothelial cell.2. The MVD of CCRCC was obvious higher than non-CCRCC.3. The expression of CD31 and CD34 is not correlated with tumor grade and stage in ChRCC and PRCC, but in CCRCC, it was negative correlation.4. Both VEGFR-2 and VEGFR-3 can serve as markers for prognosis of papillary renal cell carcinoma. Differently, VEGFR-3 is a predictor of lymph node metastasis, increased VEGFR-2 expression could predict a potential dissemination through blood vessels.5. Both PDGFR-αand PDGFR-βcan serve as markers for prognosis of papillary renal cell carcinoma. Distance metastasis was independent predictive factors of survival for PRCC patients.6. C-kit is a helpful marker to discriminate ChRCC from other subtypes of kidney cancer, as well as a prognostic predictor of ChRCC.
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