Effect Of Infiltrating Resin On Cariesleukoplakia During Orthodontic Treatment Experimental Study

Objectives In orthodontic clinical work,patients with caries leukoplakia(White Spot Lesions)on the tooth surface are often encountered and need orthodontic treatment urgently.Demineralization of enamel surface in different degrees is a common complication after orthodontic treatment.This will lead to more serious carious leukoplakia after the end of orthodontic treatment,affecting the beauty and health of teeth.At present,permeating resin is widely used in the treatment of carious leukoplakia after orthodontic treatment.However,there are few studies on the permeating resin treatment of tooth surface before orthodontic treatment.In this study,the fresh in vitro dental model was used to simulate the treatment of carious leukoplakia with penetrating resin before and after orthodontic treatment,and the changes of color,microhardness and microstructure under scanning electron microscope were observed.To find out whether preorthodontic osmosis treatment can play a role in preventing continued demineralization during orthodontic treatment and provide experimental basis for clinical work.Methods 1 Collection and storage of samples: collect 60 first premolars or second premolars that were removed due to orthodontic treatment,and require patients to be between 12 and 18 years old,and the teeth be free of caries,fluoride spots,intact teeth,normal shape,and no cracks.Dental calculus,tissue debris,etc.on the tooth surface,put the sample in saline for use.2 Preparation of the sample: use a slow slicer to separate the crown root,place it in epoxy resin and fix it,expose its lip surface,and then polish and polish it with 600,1200,2400 and 4000 mesh silicon carbide sandpaper under running water.Naturally dry,evenly coat two layers of transparent acid-proof nail polish on the surface of the tooth except the lip surface,put it in the artificial demineralizing solution at 37? for 2 weeks,and then take it out.3 Experimental grouping: 60 demineralized isolated teeth are randomly divided into 3 large groups,with 20 teeth in each group.Group A is a blank control group,which is only treated with demineralization dyeing during orthodontic treatment,and no infiltration resin treatment is performed before and after orthodontic treatment;Group B is an infiltration resin treatment group before orthodontic treatment.The tooth surface was treated with infiltration resin before the groove;Group C was the infiltration resin treatment group after orthodontic treatment,that is,the infiltration resin treatment group was performed after the orthodontic treatment was completed.4 Experimental treatment: 1)The samples of Group B are treated with infiltration resin,while Group A and Group C are not processed temporarily.2)Establish an orthodontic model: glue brackets in the center of the clinical crown of the three groups of samples.3)Demineralization and staining: 3 groups of samples were placed in 37 ? fresh Coke for 3 weeks,fresh Coke was replaced every 6 hours,twice a day,and the rest was placed in artificial saliva at 37?.4)Remove 3 sets of sample surface brackets to remove residual adhesive and smooth the tooth surface.5)Infiltrate resin treatment of samples in Group C,and save them for testing in Groups A and B.5Test: use Olympus Crystaleye spectrophotometer to perform color analysis on the samples in Group 3;Vickers hardness tester measures the enamel surface Hardness;scanning electron microscope to observe the ultrastructure of the sample.6 Statistical analysis: SPSS26.0 is used for statistical data analysis and normality test of the data.The data of this experiment all follow normal distribution.Perform statistical analysis on the three sets of data,use ± s for statistical description,use single-factor analysis of variance for color processing,and use LSD methods for further pairwise comparisons.The t-test was used to analyze the microhardness treatment of the same group,and the single-factor analysis of variance was used for the microhardness treatment of different groups,and the LSD method was used to further compare each other.The test standard ?= 0.05,P<0.05 has statistical significance.Results 1 Visual observation: Group A: the tooth surface is rough and dull,the color around the bracket is brown,and there is obvious color difference at the bonding place with the bracket bottom plate;Group B: the tooth surface is smooth and shiny,and the bracket around the bracket bottom There is a slight color difference at the joint;the tooth surface of group C is smooth and shiny,but there is still a significant color difference between the bracket and the bracket bottom plate,which is light brown(see Figure 1).2 Colorimetric results: 1)Comparison results of color differences between different parts of the same group: Group A has different colors between different parts of the tooth surface(P<0.05),and the color difference is greater than 3.7,which is within the clinically unacceptable range;There is a difference in color between different parts of the tooth surface of group B(P<0.05),the color difference value is greater than1.7 and less than 3.7,which is within the clinically acceptable range;there is a difference in color between different parts of the tooth surface of group C(P<0.05),color difference Greater than 3.7,within the clinically unacceptable range.2)Comparison of color difference between the same parts in different groups: There are differences in color between the same parts in the three groups of samples(P<0.05).3Surface microhardness test results: 1)Comparison between different parts of the same group: the microhardness around brackets in groups A,B,and C are all less than the microhardness in the bonding area of the bracket bottom plate(P<0.05).2)Comparison between the same parts in different groups: the microhardness of each part in group B and group C is greater than that in group A(P<0.05);the microhardness around brackets in group B is greater than group C(P<0.05).4 Scanning electron microscope results:Group A: Under the microscope,the structure of the enamel surface was dissolved and destroyed,and deep pits were seen,and the surface was changed in a honeycomb shape(see Figure 3).Group B: The honeycomb pores are reduced and the surface becomes flat,with only a few pores(see Figure 4).Group C: The honeycomb pores are reduced,the surface is basically flat,and there are still obvious cracks(see Figure 5).Conclusion 1 Experiments show that infiltrating resin treatment of caries leukoplakia before orthodontic treatment can resist the demineralization and staining of enamel during orthodontic treatment.2 Experiments have shown that compared with infiltration resin treatment after orthodontic treatment,infiltration resin treatment of caries leukoplakia before orthodontic treatment is better at improving color and increasing micro hardness.Figure5;Table2;Reference 92.