To Investigate The Th1/Th2 Balance Of Rats With Allergic Rhinitis Induced By Acupoint Embedding At Yingxiang Acupoint Acting On DC

Purpose:From this study Yingxiang point acupuncture point bury line of dendritic cells of rat nasal mucosa epithelial cells and the pathogenesis of AR,AR rat model was established,the grouping of Yingxiang point acupuncture point bury line,fake embedded wire and fluticasone propionate intervention,ethology score determined after intervention,and extract all the rat nasal mucosa and femoral artery blood serum.Nasal mucosa tissue pathological situation,detected by western blot detection the expression of CD80 and CD86,by ELISA detection in serum s Ig E,?IL-2?IL-4?IL-5?IL-6?IL-10 and the level of IL-12 the secretion of cytokines,explore Yingxiang point acupuncture point bury line by acting on DC and regulating Th1/Th2 balance,adjust the high reaction condition of nasal mucosa,so as to achieve mitigation and treatment of AR.Method:40 healthy SD rats were randomly divided into blank group A,AR model group B,flutekassone propionate group C,acupoint line embedding group D,and false line embedding group E,with 8 rats in each group.All the rats except the blank group were modeled with ovalbumin combined with aluminum hydroxide.After the model was successfully made,the AR model group and blank group did not intervene.Fluticasone propionate group received nasal drops of fluticasone propionate once a day for 2 weeks.Line embedding in Yingxiang point of acupoint embedding group was performed once a week for consecutive 2 weeks.False embedding line group line embedding feint,no embedding line,once a week,consecutive 2 weeks.Observe and record each related behavior in rats,by ELISA detection in serum s Ig E,?IL-2?IL-4?IL-5?IL-6?IL-10 and the level of IL-12 the secretion of cytokines,HE staining detection of nasal mucosa tissue pathology,Western blot analysis between groups in the rat nasal mucosa tissue CD80,CD86 protein expression,finally,all the results were statistically analyzed.Result:1.Results of AR related behavioral scores of rats in each group: compared with group A,the behavioral scores of group B and group E were significantly increased,which was statistically significant(P < 0.05).Compared with group B and group E,the scores of group C and group D were significantly lower(P < 0.05),and is no significant difference between the groups(P > 0.05).2.Under the light microscope,HE staining of the nasal mucosa tissues of rats in each group showed that: in group B and group E,the nasal mucosa cilia were disordered,prostrate and even exfoliated,with more lymphocytes and eosinophils,and submucosal gland hyperplasia was obvious.Compared with group B,group C and group E had less inflammation.3.The results of serum s Ig E detected by ELISA showed that the difference between group A and group C is not significant(P>0.05),but they are significantly different from group B,D,and E(P<0.05);group B and group E are not significantly different(P>0.05),there was no significant difference between group C and group D(P> 0.05).4.ELESA detection in serum IL-2?IL-4?IL-5?IL-6?IL-10 and IL-12 cytokines level: group A and group C,group B and group E,group C and D,group A and group D(not including IL-10)is no significant difference between two groups(P>0.05),group A and group B and group E difference(P<0.05),IL-10 differences between group A and group D(P<0.05).5.Western blot analysis of the expression of CD80 and CD86 proteins in rat nasal mucosal tissues: The cluster of the blank control group of group A was the same subset as the fluticasone propionate treatment group of group C and the acupoint embedding line group of group D,with no significant difference(P>0.05).The clustering of AR model group B and false embedded line group E was the same subset,and the difference was not significant(P>0.05).The differences between the remaining groups were significant.Conclusion:1.Acupoint catgut embedding at Yingxiang could significantly improve the symptoms of AR rats,reduce serum s Ig E and reduce the inflammatory response of nasal mucosa,and there was no significant statistical difference compared with the treatment of fluticasone propionate(P>0.05).2.Embedding wires at Yingxiang acupoint can reduce the expression levels of CD80 and CD86 proteins in AR rats,reduce the levels of s Ig E and IL-4,IL-5 and IL-6,and increase the levels of IL-2?IL-10 and IL-12,so as to correct the imbalance of Th1/Th2 in the body,eventually relieve the clinical symptoms of AR and achieve the purpose of treatment.