Screening And Analysis Of Long Non-coding RNA Differently Expressed In Patients With Papillary Thyroid Carcinoma

Objective: To investigate the pathogenesis of papillary thyroid carcinoma,This study used second-generation sequencing technology to screen differentially expressed lnc RNAs in thyroid papillary carcinoma tissues,and to explore its potential functions in the occurrence and development of papillary thyroid carcinoma.Providing more sufficient theoretical basis for the prevention,diagnosis and treatment of PTC.Method: According to strict inclusion and exclusion criteria,specimens of cancer tissues and adjacent tissues from 5 patients with papillary thyroid carcinoma were collected,and lnc RNA expression profiles were detected by high-throughput sequencing technology.Target genes were predicted for lnc RNAs with expression differences greater than 5 times.And the GO enrichment analysis and KEGG pathway analysis were further performed.Results: There were a large number of differentially expressed lnc RNAs and mRNAs in papillary thyroid carcinoma tissues and adjacent tissues.A total of 2444 differentially expressed lnc RNAs were selected in this study,of which 1707 were up-regulated and 737 were down-regulated.A total of 3983 mRNAs were differentially expressed that were more than 5 times,including 2228 up-regulated and 1755 down-regulated.The target genes of these differentially expressed lnc RNAs were predicted,and it was found that there were 195 up-regulated lnc RNAs and 100 down-regulated lnc RNAs with target genes.The GO enrichment analysis showed that the function most strongly associated with differentially up-regulated lnc RNAs was protein serine / threonine kinase activity;the function most strongly associated with differentially down-regulated lnc RNAs was DNA binding transcription activator activity,RNA polymerase II specificity.Analysis of the KEGG pathway revealed that the most obvious up-regulated signaling pathway was the pentose phosphate pathway,and the most obvious down-regulated signaling pathway was glycerophospholipid metabolism.Conclusion: There are a large number of differentially expressed lnc RNAs in papillary thyroid carcinoma,which may participate in the occurrence and development of papillary thyroid carcinoma through certain signaling pathways.