Experimental Studies On The Expression Of MMP-1,MMP-3,EGFR MRNA In The Aging Skin Of Mice With Lycium Ruthenicum Procyanidins

Objective: To study the intervention effect of Proanthocyanidins from Lycium ruthenicum,on the aging skin model of mice induced by ultraviolet(UVA+UVB)irradiation combined with subcutaneous injection of D-galactose.The relative expressions of matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-3(MMP-3)and epidermal growth factor receptor(EGFR)in skin tissue of mice were determined by RT-q PCR.The effect of vegetable on aging skin in mice.Methods: 50 healthy SPF Kunming female mice were randomly divided into 5 groups: 10 in normal control group(A),10 in model group(B),and 10 in positive control group(C).10 in low-dose of proanthocyanidins from Lycium ruthenicum Group(D),10 in high-dose of proanthocyanidins from Lycium ruthenicum Group(E);establishe the mouse aging model;RT-q PCR was used to determine the m RNA expression levels of MMP-1,MMP-3 and EGFR in skin tissues of each group.Results: Compared with the model group,the relative expression of MMP-1,MMP-3 and EGFR in the skin tissue of the normal control group was significantly decreased,the difference was statistically significant(p<0.05);compared with the model group,the expression of MMP-1,MMP-3 and EGFR in the skin tissue of mice in the positive control group and the low-dose group of proanthocyanidins from Lycium ruthenicum decreased significantly,the difference was statistically significant(p<0.05),and the low dose of proanthocyanidins in the Lycium ruthenicum,the relative expression of the group was lower than that of the positive control group,and the difference was statistically significant(p<0.05);the relative expression of MMP-1,MMP-3 and EGFR in skin tissue of mice in the high-dose group of Lycium barbarum proanthocyanidins was significantly lower than that in the low-dose group,the difference was statistically significant(p<0.05).Conclusion: Lycium ruthenicum proanthocyanidins can reduce the expression levels of MMP-1,MMP-3 and EGFR in aging skin tissues of mice,and the reduction level is greater than that of the same dose of vitamin E intervention.It shows that Lycium ruthenicum proanthocyanidins is a strong antioxidant and has certain effect in delaying skin aging.