The Relationship Between Gene Polymorphism Of Osteoprotegerin Promoter Region And Vascular Calcification In Coronary Artery Disease

Objectives The distribution of 950T/C and 149T/C genes in the promoter region of osteoprotegerin(OPG)was statistically analyzed in normal control group,coronary artery disease(CAD)group,coronary artery calcification group and non-vascular calcification(VC)group.To investigate the relationship between serum osteoprotegerin level and promoter gene polymorphism and coronary artery disease and vascular calcification.Method From April 2015 to October 2018,482 patients with coronary angiography were selected and divided into normal control group(148 cases)and coronary artery disease group(334 cases).The coronary artery disease group included 150 cases of calcification group and 184 cases of non-calcification group.The age,sex,smoking history,past history of hypertension?diabetes mellitus and other general clinical data were recorded.The fasting venous blood was collected and sent to the biochemical room the next morning.Blood glucose,blood lipid,blood routine,liver and kidney function,myocardial enzyme and coagulation function were detected.Wait for many indexes,and record the results of the test.By using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technique,DNA,was extracted from the blood of the study subjects to understand the osteoprotegerin genotype in the normal control group,coronary artery disease group and coronary artery calcification group.To determine the relationship between the polymorphism of 950T/C and 149T/C sites in the promoter region of osteoprotegerin and coronary heart disease and vascular calcification in patients with non-vascular calcification.In addition,66 cases of normal control group,73 cases of non-calcification group and 86 cases of calcification group were randomly selected to detect their blood by enzyme-linked immunosorbent assay(ELISA).The levels of osteoprotegerin(OPG),soluble nuclear factor ? B receptor activating factor ligand(sRANKL),matrix metalloproteinase-9(MMP-9),monocyte chemoattractant protein-1(MCP-1)were observed in normal control group,coronary artery disease group and coronary arterycalcification group,non-vascular calcification group.Results 1.There were significant differences in sex,age,smoking history,diabetes history and HDL-C,ApoA1,ApoA1/ApoB,LP(a),CK,TNT,Hcy,UA between the control group?the CAD group and CAD calcification group(P<0.05),but there was no significant difference in the family history of coronary artery disease,the history of hypertension and TG,TC,LDL-C,ApoB,FBG,CK-MB,ALP between the groups(P> 0.05).2.There were significant differences in age,LP(a)between coronary artery calcification group and non-vascular calcification group(P<0.05),but in sex,smoking history,diabetes history,hypertension history,family history of CAD,TG,TC,LDL-C,HDL-C,ApoA1,ApoA1/ApoB,FBG,CK-MB,CK,TNT,Hcy,UA,ALP,there was no significant difference between the two groups(P>0.05).3.In this study,we found that there are three types of gene types of 950T/C and149T/C in the promoter region of OPG,among which TC genotype was most common in 950T/C locus,TT genotype was in the middle,CC genotype was the least,T allele was most common and C allele was few.TT genotype was the most common genotype of 149T/C locus,TC genotype was middle,CC genotype was the least,T allele was most common and C allele was less.4.There were significant difference in genotype distribution of 950T/C locus between control group and CAD group?CAD calcification group(P<0 05),there was no significant difference between calcification group and non-calcification group(P>0.05).There was no significant difference in genotype distribution of 149 T / C locus between control group and CAD group,and between calcified group and non-calcified group(P>0.05).5.Compared with the control group,the serum OPG,sRANKL level was higher and the serum MMP-9,MCP-1 level was lower in the CAD group,but there was no significant difference between the two groups(P>0 05).Compared with the non-calcified group and the control group,the serum sRANKL level in the calcified group was significantly higher(P<0.05).The increase of serum OPG,MMP-9,MCP-1 level was not statistically significant(P>0.05)Conclusion 1.Factors related to the occurrence and development of CAD are sex,age,smoking history,diabetes history,HDL-C,ApoA1,ApoA1/ApoB,LP(a),CK,TNT,Hcy,UA.The main factors related to vascular calcification are age,LP(a).2.The gene polymorphism in the promoter region of OPG is related to the occurrence and development of CAD and coronary artery calcification.950T/C genotype distribution is associated with coronary artery disease and coronary artery calcification.However,the genotype distribution of 149T/C locus had no significant correlation.Serum sRANKL level is related to coronary artery calcification,indicating that OPG system is involved in the disease progress of AS,vascular calcification,suggesting that serum OPG,sRANKL may be used as a new biomarker to predict the prevalence and severity of coronary artery disease.