Effect Of Di-(2-Ethylhexyl)phthalate(DEHP)in BALB/c Mice Of Allergic Rhinitis

Objectives: Allergic rhinitis(AR)is a common chronic inflammatory disease of the upper respiratory tract.Di(2-ethylhexyl)phthalate(DEHP)is a widely used plasticizer and belongs to environmental endocrine disruptors(EEDs).It can be entered the human body which is harmful to health.This study aims to investigate the effect of environmental pollutants DEHP on AR by detecting the inflammatory expression of serum and nasal mucosa and research the expression of aryl hydrocarbon receptor(AhR)and its related pathways in AR mouse model.Methods: 24 BALB/c mice were used as the study subjects,and ovalbumin(OVA)and DEHP were used for modeling.They were divided into control group,DEHP group,OVA group and OVA+DEHP group.Studying by pathological examination,allergic symptom scores,ELISA testing,oxidative stress testing,detection of AhR and signaling pathways CYP1A1 and CYP1B1 related proteins and mRNA.Results: HE-staining showed that many chronic inflammatory cell infiltration and nasal mucosal destruction were observed in the OVA+DEHP group and were more severe than the OVA group.Allergic symptom scores were obtained from sneezing,scratching,number ofscratching,and nose flow.The scores of the OVA group and the OVA+DEHP group were higher than 7 points.Serum ELISA tests are more serious in the OVA+DEHP group.And by detecting oxidative stress in nasal mucosa,the activities of glutathione catalase and superoxide dismutase in DEHP,OVA and OVA+DEHP groups were decreased compared with NC group.And compared with OVA group,the decrease in activity was more pronounced in OVA+DEHP group.The expression of reactive oxygen and malondialdehyde increased,which was statistically significant.The expression of AhR protein and its mRNA was increased in the DEHP group,OVA group and OVA+DEHP group.The OVA+DEHP group was more significant than the OVA group.And the mRNAs of the AhR-related signaling pathways CYP1A1 and CYP1B1 were also more prominent in the OVA+DEHP group.Conclusions: DEHP may aggravate its inflammatory response through the AhR pathway closely related to the environment.When combined with OVA,DEHP can further aggravate the OVA-induced nasal inflammatory response and make the nasal cavity and the body have undergone severe changes,and many inflammatory cells have infiltrated.DEHP has shown an adjuvant effect,and the AhR-related signaling pathways CYP1A1 and CYP1B1 may be associated with it.