| Background&purpose:The impact of air pollution on human health has been intensively studied.Most studies mainly focus on their adverse effects on the respiratory system and the cardiovascular system.However,the skin as the body’s largest organ,is also the interface between the body and various air pollutants,very little research is available on the impact of air pollution on skin.In this study,we investigated the role of AhR in melanogenesis when the environmental pollutants PAHs and PHAHs were exist and we tried to weaken this effect.Methods:TCDD,benzo(a)pyrene,(3-arbutin and resveratrol four reagents were treated separately or mixed into B16-F10 cells.We used CCK-8 experiments to determine the toxicity of reagents to cells.Total melanin content and tyrosinase activity were detected,the effect of reagents on melanogenesis-related genes and AhR at the transcriptional stage was analyzed by Real-time PCR and the effects on AhR protein were measured by Western blot.Results:CCK-8 experiments:The optimum concentration range of different reagents were determined.Arbutin 20 μg ·mL-1,resveratrol 8 μg ·mL-1,benzopyrene 10 μg ·mL-1 and TCDD 50 ng· mL-1 were used for subsequent experiments.Total melanin content and tyrosinase activity experiments:The addition of(3-arbutin and resveratrol could effectively reduce the increase of melanin content and tyrosinase activity caused by B[α]P and TCDD.Real-time PCR and Western blot experiments:β-arbutin and resveratrol can down-regulating the transcription level of AhR,TYRP-1 and TYRP-2,while benzopyrene and TCDD has opposite effect.The effect of mixed reagents on the transcription level of AhR was between two pure reagents.Consistent with the conclusion of total melanin content and tyrosinase activity experiments.Western blot indicate that the effect of reagent on AhR protein was consistent with that of transcription level.Conclusion:AhR is likely to play a role in melanogenesis by regulating the expression of melanogenic genes.Arbutin and resveratrol may have an effect on the reduction of melanogenesis caused by AhR activation. |
