The Abnormal Expression Of SIRTs In Psoriasis

?Objective?Study the abnormal expression of SIRTs in psoriasis to lay a theoretical foundation for the clinical treatment and prognosis of psoriasis.?Methods?1.Animal model: the treatment group and the control group,BABL / c mice were treated with 5% imiquimod cream(Ming xin Li di)or normal saline for 7 days.All mice were sacrificed on day 8 and collected the mouse back skin tissue.2.Cell experiments: TNF-? induced HaCaT cells.3.Clinical severe psoriasis(PASI> 10)fresh specimens and benign specimens were collected according to the age,gender and sample location of the patients.4.The mRNA and protein expressions of SIRTs were detected using real-time PCR and western blotting compared with the control group.5.Immunocytofluorescense examination was designed to study the specific expressions and locations of SIRT family proteins in cells.6.Statistical analysis.?Results?1.Mice psoriasis model showed significant erythema,scales and inflammation on the back of the BABL/c mice.2.The results of TNF-?-induced HaCaT cells showed the intuitive changes,such as the morphology and intercellular space.3.qRT-PCR was used to evaluate the mRNA expression level of SIRTs.mRNA expression level of SIRT1,SIRT2,SIRT3,SIRT4 and SIRT5 were decreased,however,mRNA expression level of SIRT6,SIRT7 were on the rise,which were also confirmed by western blotting and immunofluorescence.4.Another function of immunofluorescense is SIRTs localization.SIRT1 and SIRT2 not until express mainly in cytoplasm but also nucleu.SIRT3,SIRT4,and SIRT5 are primarily mitochondrial proteins.However,SIRT6 and SIRT7 are nuclear sirtuins.What more,the binding site of SIRTs antibody were mainly based on the epidermis according to the staining results of immunofluorescence.?Conclusion?SIRTs family plays significant roles in psoriasis dermatitis and TNF-? induced HaCaT cells with the over-expression of SIRT6 and SIRT7 and the down-regulation of SIRT1,SIRT2,SIRT3,SIRT4 and SIRT5.