Correlation Between Oral Squamous Cell Carcinoma And Human Papillomavirus

Background: More than 95% of oral cancers are squamous cell carcinomas.Tobacco,alcohol,bad stimuli,etc.are recognized causes,but studies have observed that approximately 15-20% of patients are not exposed to recognized risk factors,suggesting that other possible risk factors exist.The hypothesis that HPV infection was one of the possible causes was first proposed in 1983,and there are related studies in the follow-up.However,the role of HPV in the development of oral squamous cell carcinoma is still unclear.This paper will discuss this issue.Methods: A total of 100 new cases of oral squamous cell carcinoma diagnosed by pathology were obtained from the Stomatology of the Fourth Hospital of Hebei Medical University.The healthy group consisted of 100 healthy people in the community.The general characteristics of the healthy group were consistent with the case group.The cell brush kit was used to collect the exfoliated cells of the oral mucosa.The case group used a cell brush to cover the mucous membrane around the oral cavity.The range was about 2-3 cm,rotated 3 times clockwise,and then rotated 3 times clockwise in the lesion area.Put into the specimen tube containing the cell preservation solution,the cap is screwed and marked and immediately sent for HPV typing.In the healthy group,the scraping range was bilateral cheeks,tongue back,and upper sacral mucosa.The sampling method was the same as above.Synchronous nucleic acid amplification and detection were performed by a real-time fluorescent PCR instrument using a multiplex fluorescent PCR technique to detect a type 23 HPV type.Results: One of the 100 oral squamous cell carcinoma patients with oral exfoliated cells was positive for HPV,and the HPV positive rate was 1.0%.The HPV fluorescence PCR results of oral exfoliated cells in 100 healthy subjects were all negative.Between the case group and the healthy group,there was no significant difference in the HPV positive rate.Conclusion: The oral HPV infection rate in healthy people and oral squamous cell carcinoma patients in this study is low,which can not support the important role of HPV in the development of oral squamous cell carcinoma.HPV detection for early screening of oral squamous cell carcinoma and The application of early prevention still needs more research.