The Infection Of Human Papillomavirus In Chinese Patients With Oral Potentially Malignant Disorders And Oral Squamous Cell Carcinoma

Objective: The critical role for HPV in OPSCC has been confirmed,but the role for HPV in oral squamous cell carcinoma(OSCC)and oral potentially malignant disorders(OPMD)are still controversial.The aim of this study was to verify HPV detection rate in Chinese OSCCs and OPMDs patients.Materials and Methods: Paired tissue and serum DNA samples were extracted from 59 OPMD and 40 OSCC patients.A Sybr Green-based real-time PCR assay was developed utilizing primers to the highly specific E6 gene detecting HPV16 and HPV18.Sequenc analysis and PCR-reverse dot blot(PCR-RDB)assay were further performed to verify real-time PCR results and detect other 21 HPV subtypes,including HPV 6,11,31,33,35,39,42,43,45,51,52,53,56,58,59,66,68,73,81,82 and 83.Results: We found that none of the tissue and serum samples of OPMDs and OSCCs were positive for HPV16 E6 or 18 E6 DNA.Overall,3 of 198(1.52%)and 7 of 198(3.54%)samples were false-positive for HPV16 E6 and HPV18 E6,respectively,using real-time PCR.PCR-RDB assay not only confirmed real-time PCR data,but also showed negative infection of HPV 6,11,31,33,35,39,42,43,45,51,52,53,56,58,59,66,68,73,81,82 and 83 subtypes.Conclusion: Lack of HPV16 and HPV18 detected in this study implies that high risk HPV infection was not a frequent event in OPMDsand OSCCs of Chinese patients studied.Real-time PCR followed by DNA sequencing or PCR-RDB assay for HPV DNA detection is an effective strategy to rule out false positives.