Research On Nanofat Assisted Fat Grafting

Soft tissue augmentation due to congenital malformation,trauma,tumor resection and cosmetic requirement is an increasingly popular topic in plastic surgery.Autologous adipose tissue is one of the most ideal filler in the treatment of soft-tissue defects.It is readily available,easily obtainable and biocompatible,and causes little damage to the donor site.However,the high absorption and low survival rate of grafts following transplantation is a major concern,which limits the wide clinical application of fat graft.Nanofat is an emulsified suspension derived from fat tissue that is processed by purely mechanical means.Previous studies have demonstrated that nanofat contains a large amount of mesenchymal stem cells,and revealed its regenerative capacity in clinical application.Objective:The aim of this study was to explore the effect of nanofat on the long-term retention of fat grafts and its possible mechanism.Methods:Fat grafts were transplanted subcutaneously into nude mice with different nanofat volumes or without nanofat.The grafted fat was dissected 12 weeks after transplantation.Graft weight and volume measurement and histological evaluation were performed to evaluate the survival of fat grafts.In addition,capillary density and angiogenesis-related gene expression were measured to explore the possible mechanism preliminarily.Result:The co-transplantation of fat with nanofat showed higher graft weight and volume retention,better histological structure,higher capillary density and higher angiogenesis-related gene expression compared to that in controls.However,there were no significant differences between the two nanofat volumes utilized.Conclusion:Nanofat improves fat graft survival,possibly via enhancing the angiogenesis.