Fat Extract Improves Random Pattern Skin Flap Survival In A Rat Model

Backgroud In early ischemic and hypoxic environment,angiogenesis is one of the keys to improve the survival of random skin flap.Adipose tissue and its derivatives promote angiogenesis and tissue regeneration by adipose stem cells and extracellular matrix.Nanofat is rich in adipose stem cells,extracellular matrix and growth factors,obtained from mechanical emulsification of fat tissue,which also contains strong proangiogenic capacity.Objectives The aim of this study is to establish a protocol for the isolation of fat extract(FE)from nanofat and to evaluate the effects of FE on the skin flap survival in a rat model.Method 1.Investigate the effect of emulsification times on the preparation of nanofat.2.Purify FE from nanofat.3.Analyze the total protein and levels of growth factors in FE by BCA and ELISA.4.Evaluate the effects of FE on cell proliferation,migration and tube formation of human umbilical vein endothelial cells(HUVECs)in vivo.5.Evaluate the efficacy of FE on the survival of random skin flap in a rat model.Results 1.After 60 times of emulsification,increasing the emulsification times does not enhance the efficacy of nanofat production.2.FE contained none of cell and oil fraction.3.FE was enriched with variety of growth factors.4.FE promoted the cell proliferation,migration and tube formation in HUVECs in vitro.5.FE enhanced the survival of rat random skin flap via increasing angiogenesis.Conclusion FE is a cell free fat derivative and enriched with variety of growth factors.The proangiogenic activity of FE has been demonstrated both in vitro and in vivo studies.Attribute to the simple preparation with none chemical addition and convenience of storage,FE could be potential used in clinical applications.