| Objective:Type 2 diabetes mellitus?T2DM?is a metabolic disease with elevated blood glucose.It is characterized by insulin resistance and hyperglycemia caused by islet?-cell dysfunction,and its etiology and pathogenesis have not yet been fully elucidated.Diabetic macrovascular complications are the main cause of the deterioration or even death of patients.Their development is slow,the onset is concealed,and it is difficult to diagnose and treat them early.Once it happens,it will be difficult to reverse,which will have a serious impact on the social economy and the quality of life of patients.A large class of diseases that threaten human health.It has been confirmed that the risk of cardiovascular and cerebrovascular disease is higher in the T2DM population than in the non-diabetic population,and is also the leading cause of death and disability in T2DM patients.Carotid intima-media thickness?CIMT?is an important indicator for early evaluation of atherosclerosis and has important predictive value for the occurrence of atherosclerotic cardiovascular and cerebrovascular events.The mechanism of diabetic macroangiopathy has not been thoroughly studied.There is no similar study on the relationship between miR-26a and early atherosclerosis in diabetic patients.To investigate the expression of miR-26a in serum of patients with type2 diabetes mellitus and the relationship between plasma miR-26a level and carotid intima-media thickness?CIMT?in patients with T2DM.Method:1.96 patients with well-diagnosed T2DM who were admitted to Linyi City People's Hospital from October 2017 to June 2018 were selected as subjects.Carotid color ultrasound was used to measure CIMT.CIMT<0.9 mm was normal for CIMT.Group?n=49?,CIMT?0.9 mm was CIMT thickening group?n=47?,healthy control group 30cases?n=30?,all were selected in our hospital physical examination center,physical examination was normal.2.Carotid intima-media measurement method:carotid intima-media thickness refers to the distance between the upper edge of the carotid intima and the medial-outer interface,and the distal bifurcation of the common carotid artery is selected.The posterior wall of 3cm at 1 cm above the fork and 1 cm above the beginning of the internal carotid artery was measured and the average of the bilateral measurements was taken.According to the"2010 Guidelines for the Prevention and Treatment of Hypertension in China",the definition of target organ damage was determined by carotid color ultrasonography CIMT0.9 mm.3.Related metabolic indicators and general data collection:detection of fasting blood glucose?FPG?,fasting C-peptide?FC-P?,glycosylated hemoglobin?HbA1c?,blood pressure?BP?,triglyceride?TG?,total cholesterol?TC?,Low density lipoprotein cholesterol?LDL-C?,high density lipoprotein cholesterol?HDL-C?,creatinine?SCr?.Carefully register body mass index,gender,age,current medical history,past history,family history,drinking status,etc.4.Real-time quantitative polymerase chain reaction?qRT-PCR?was used to detect the expression level of plasma miR-26a in all subjects,and other metabolic indicators were determined.5.Statistical analysis:Compare the expression levels of plasma miRNA-26a between the three groups,analyze the correlation between the expression level of plasma miRNA-26a and related metabolic indicators,and explore the clinical expression of plasma miRNA-26a in type 2 diabetes.Significance,and the relationship between plasma miR-26a levels and carotid intima-media thickness?CIMT?in patients with T2DM.Result:1.Comparison of three groups of baseline data and biochemical indicators:gender,age,BMI,duration of disease,systolic blood pressure,and diastolic blood pressure were not statistically significant?P>0.05?,which was balanced and comparable;compared with healthy controls.The serum levels of HbA1c,FPG,FC-P,TC and LDL-C in CIMT normal group and CIMT thickening group were significantly increased?P<0.05?.Compared with the control group,serum TG increased in the normal CIMT group.The difference was statistically significant?P<0.05?.2.The expression level of miR-26a in type 2 diabetes group and control group:Compared with the control group,the expression level of plasma miR-26a in T2DM group was significantly?1.241±0.724,0.046±0.946?,the difference was statistically significant?P<0.05?.3.Comparison of three groups of miR-26a expression The expression level of plasma miR-26a in CIMT thickening group and CIMT normal group was significantly higher than that in healthy control group[?1.540±0.481,0.967±0.802 vs.0.046±0.945?,both P<0.05]The expression level of plasma miR-26a in CIMT thickening group was further higher than that in normal CIMT group,and the difference was statistically significant?P<0.05?.The differences between the three groups were statistically significant?F=36.29,P<0.01?.4.Correlation analysis between miR-26a and various indicators:Pearson correlation analysis showed that plasma miR-26a expression was positively correlated with FPG,HbA1c,TC and LDL-C?r=0.308,0.433,0.195,0.190,P<0.05?,negatively correlated with FC-P?r=-0.410,P<0.05?,and had no correlation with age,disease duration,BMI,SBP,DBP,TG.5.Multivariate stepwise regression analysis of factors affecting miR-26a expression:miR-26a as a dependent variable,FPG,HbA1c,FC-P,TC,TG,LDL-C,HDL-C,Scr,age,duration,BMI Multivariate linear regression analysis as an independent variable showed that HbA1c and FC-P were independent factors of miR-26a?r=0.095?-0.223,P<0.05?,and the regression equation YmiR-26a=0.39+0.095 HbA1c-0.223 FC-P.Conclusion:The expression level of plasma miR-26a is increased in the control group,CIMT normal group and CIMT thickening group.The expression level of plasma miR-26a is related to FPG,HbA1c,TC and LDL-C.HbA1c and FC-P are independent factors influencing miR-26a.miRNA-26a is closely reated to type 2 diabetes and carotid intima-media thickness. |
PDF Full Text Request