The Correlation Analysis Between Plasma HMGB1and Carotid Intima-media Thickness/Plaque

Objective:To explore the risk factors of carotid intima-media thickness (c-IMT)thickening and carotid plaque formation as well as the possible treatments for futureuse through the analysis of plasma high mobility group box-1protein(HMGB1)concentration in subjects with or without c-IMT thickening and the correlationanalysis between HMGB1and c-IMT thickening/carotid plaque formation. Methods:We selected67cases with c-IMT thickening as thickening group and24cases withoutc-IMT thickening as control group among subjects in patient, and further grading thec-IMT thickness as well as presence or absence of carotid plaque formation.Meanwhile, we analyzed the correlation between HMGB1and c-IMT thickening/carotid plaque formation through comparing the plama HMGB1using enzyme-linkedimmunoassay (ELISA) upon the basis of somatometric measurement and generallaboratory examination. Results:?There were no statistical differences in age, sex,BMI, BP and WBC counts (all P>0.05) but significantkly statistical differences inApo-B, Apo-A1, Triglyceride, total Cholesterol, High/Low density lipoprotein(HDL/LDL) cholesterol, hc-CRP (all P <0.05).?There were statistical differencesin the levels of plasma HMGB1concentration among groups (control group,15.27±2.91ng/ml; mild group,22.11±1.65ng/ml; moderate group,27.63±2.80ng/ml and severe group,32.18±3.38ng/ml, respectively; all P <0.0001).Meanwhile, there were statistical differences in the HMGB1level with regards to theformation of carotid plaque (no plaque group,19.25±4.29ng/ml; plaque group,29.22±4.86ng/ml, respectively; all P <0.0001).?Multiple linear regressionanalysis showed that the risk factors for c-IMT thickening were HMGB1, HDL-Cho,TG, Apo-A1, hc-CRP and HMGB1was the major risk factor with standardizedregression coefficient(0.864, less than0.25for others). A partial correlation coefficient(0.298; P=0.008) was obtained between the HMGB1level and c-IMT thickness afteradjustment for others factors. There was a strong positive correlation between theHMGB1level and c-IMT thickness using linear correlation and regression analysis (Pearson correlation coefficient=0.91; coefficient of determination R2=0.83; P <0.0001).?HMBG1was the independent risk factor for carotid plaque formation(OR=2.247,P=0.003) with carotid plaque formation as the dependent variable(standard logistic regression coefficient=2.956), indicating the significant influenceof HMBG1on the carotid artery plaque formation. Conclusion:?There arestatistical differences in the levels of plasma HMGB1concentration among subjectswith or without c-IMT thickening, and HMGB1is the independent risk factor forc-IMT thickening. Meanwhile, a strong positive correlation exsits between theHMGB1level and c-IMT thickness. Furthermore, c-IMT thickness is increasing alongwith the increasement of plasma HMGB1concentration.?Blood HMGB1iscorrelate with the formation of carotid plaque and is the independent risk factor forthe formation of carotid plaque. There is statistical difference in the HMGB1levelsamong subjects with or without carotid plaque formation.