| Hawthorn is a dry mature fruit of the red Crataeguspin,natiida Bge.var.major N.E.Br.or Crataeguspin.natifida Bge.in the Rosaceae plant.Hawthorn is the representative medicine for the method of clearing and frying in traditional Chinese medicine processing,and it is also one of the most medicinal materials that can reflect the concept of "firepower".The products of Hawthorn are divided into raw hawthorn(clean hawthorn),fried hawthorn,Charred hawthorn,and hawthorn charcoal.The concoction is different,and its efficacy and indication are not the same.Most of the judgments on the heat of the hawthorn are made subjective judgments by the human eye to observe the changes in the characteristics of the hawthorn during the frying process,and there is no objective standard.In the Chinese Pharmacopoeia,the identification of different processed products of Hawthorn is differentiated only by the level of organic acid(based on citric acid).Can not reflect the difference between the characteristics of the processing and the efficacy.In order to further study the effects of the frying process on the traits and internal components of Hawthorn pieces,the method of Hawthorn processing was carried out to improve the curative effect of traditional Chinese medicine decoction pieces.This study carried out the changes of traits,HPLC profiles and pharmacodynamic effects during the frying process of Hawthorn.the study.This study first used traits as entry points.The self-developed machine vision system was used to segment and analyze the color of the flesh part of the process of Hawthorn processing,and the change law was studied,and finally it was presented in the form of objective figures.The color range of the fleshy part of the hawthorn is L*(101.599~147.994),a*(142.393~150.048),b*(138.127~157.956);the color range of the fleshy portion of fried hawthorn is L*(46.334~137.408),a *(134.801~152.431),b*(132.420~157.902);the color range of Charred hawthorn is L*(17.971~114.575),a*(127.154~149.617),b*(127.189~154.776);the color range of Hawthorn charcoal It is L*(12.139~54.008),a*(126.560~142.376),b*(126.290~139.861);the color range of carbonized hawthorn with excessive heat is L*(10.778~35.099),a*(126.335~129.663)b*(126.434~129.154),successfully realized the objective description of different processed products of Hawthorn from the color angle;in addition,this study used the Heracles II type fast gas phase electronic nose to study the odor change law during the processing of Hawthorn,and change it.The more obvious components use the Arochembase database of the gas phase electronic nose system to analyze the odor characteristics,and explore the odor change law during the processing of the hawthorn,and finally use a variety of mathematical models,including Discriminant Factor Analysis(DFA)and Principal Component Analysis(PCA)have successfully realized the effective differentiation and identification of different processed products of Hawthorn based on odor information.Since then,we have further carried out HPLC fingerprint analysis of the hawthorn processing process.It was found that the composition of the HPLC spectrum during the scorching process of the hawthorn produced significant changes.The content of the original active ingredients such as hyperoside and chlorogenic acid decreased to varying degrees with the prolonged processing time.But at the same time,some new products,such as 5-hydroxymethylfurfural,are also produced.During the processing of hawthorn,the active constituents such as flavonoids and organic acids will be reduced to different extents,but the function of hygienic hypnosis after scorching will be enhanced.The internal mechanism is still unclear.Therefore,this study first verified the function of "longer than digestive stagnation" of Hawthorn,and found that Charred hawthorn did promote the intestinal propulsion rate and gastric emptying rate of atropine mice.In order to explore the underlying mechanism,we successfully established a functional dyspepsia(FD)rat model using the tailstimulation method,and then intervened with the drugs of raw hawthorn and pyrophyllum,and normal rats and model rats.Intestinal flora was analyzed by intervention of Hawthorn and rats with Charred hawthorn.The study found that compared with normal rats,the relative abundance of Helicobacter pylori and polymorphic bacteria in the intestinal tract of FD model rats was higher,but the relative abundance of Bacteroides,Lactobacilli and unclassified Helicobacter pylori was lower.The abundance levels of the above-mentioned flora were restored to different degrees after the intervention of Hawthorn and Charred hawthorn,and the recovery degree of the Charred hawthorn intervention group was more obvious.This may be one of the internal mechanisms that the hawthorn is more prone to "digestion and depression" after scorching. |
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