| Background Magnesium and magnesium alloys are close to the human body in density and elastic modulus,and medical magnesium and its alloys have good degradability.They can be naturally degraded as bone internal fixation materials,without secondary It has excellent bioactivity and can provide a good mechanical environment in the early stage of fracture healing and is easy for bone tissue growth.However,the corrosion resistance of magnesium is poor,the degradation rate in the implant is fast,it is difficult to achieve a good fixation effect,and a large amount of hydrogen is generated during the corrosion process,resulting in premature loss of biological function of the implant.The magnesium alloy is surface-treated by micro-arc oxidation technology to ceramize the surface of the magnesium alloy(AZ31)to improve the surface corrosion resistance,and to enhance the biocompatibility of the magnesium alloy through subsequent treatment.In this experiment,after micro-arc oxidation-silane/phytic acid treatment on the surface of magnesium alloy,different concentrations of HA were doped to study the effect on the biocompatibility of osteoblasts,and the surface morphology and surface elements of the test piece were observed by SEM.The method was applied to evaluate the possibility of applying the micro-arc oxidation doped HA silane/phytic acid composite film to the surface treatment of magnesium implant materials,and provide a theoretical basis for clinically available medical implant materials.Objective The surface of the magnesium alloy was subjected to micro-arc oxidationsilane/phytic acid double coupling treatment,and then doped with different concentrations of HA.The surface morphology and surface elements of the test piece were observed by scanning electron microscopy.The effect on the biocompatibility of osteoblasts.Methods First,the magnesium alloy was subjected to micro-arc oxidation-silane/phytic acid treatment as a control group A,and 2,4,and 6g/L HA were added as the B,C,and D experimental groups,respectively,based on the control group A.Four sets of test pieces were observed by scanning electron microscopy to observe the surface morphology of the test pieces and to analyze the surface elements.Then MC3T3-E1 mouse osteoblasts were used,and the cells were inoculated on the surface of each group.The cell adhesion and proliferation were detected by CCK-8 test at different time.ALP detected the alkaline phosphatase activity of cells at different time.Laser confocal microscopy was used to detect early cytoskeletal morphology.Results Under the scanning electron microscope,the surface of the treated specimen was observed.Only the micro-arc oxidation-silane/phytic acid treatment specimen was used,and there were still some tiny voids on the surface of the membrane.After the addition of different concentrations of HA,the membrane layer was the microvoids are further filled by the HA,the pores are reduced,and the surface of the test piece becomes relatively flat.As the doping HA concentration increases,the micropores are filled as much as possible.From the elemental analysis point of view,the surface of the test piece with added HA has a high content of Ca and P elements.The results of CCK-8 and ALP showed that the proliferation,adhesion and activity sequence of the surface cells of the four groups were better than those of group D,which was better than group B,and group C had the best osteoblast compatibility.Under the confocal laser confocal observation,the cell growth and extension of the surface of each group were the best in group C,the second in group B and D,the lowest in group A.According to statistical analysis,the data of the four groups of experiments were statistically significant.Conclusions The magnesium alloy micro-arc oxidation-silane/phytic acid HA treatment has achieved a good sealing effect,and the formed coating is beneficial to cell proliferation,adhesion and differentiation,and has good biocompatibility.When the concentration of HA reaches 4 g/L,the coating has the best cell compatibility. |
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