Biocompatibility Of The Composite Coating On Pure Magnesium- Ultrasonic Micro Arc Oxidation-phytic Acid-salvianic Acid A Sodium

Objective : In order to improve the corrosion resistance and cellular biocompatibility of pure Magnesium,we construct a new composite material using ultrasonic micro-arc oxidized magnesium to carry salvianic acid A sodium coupled by phytic acid.Methods : The materials are grouped as followed,A,ultrasonic micro-arc oxidized pure magnesium;B,ultrasonic micro-arc oxidized pure magnesium-phytic acid;C,ultrasonic micro-arc oxidized pure magnesium-phytic acid-salvianic acid A sodium.First,the morphology and surface element of samples are observed using scanning electron microscope(SEM).Next,we test the cellular biocompatibility using mouse osteoblast cell line MC3T3-E1.Cell adhesion and proliferation are detected on the material in each group using cell counting Kit-8(CCK-8).Finally,we use confocal microscope to characterize cytoskeleton arrangement in each group.Results : The scanning electron microscope and the coating of spectrum display was loaded successfully.Group C presents the most cellular biocompatibility,including optimal cell proliferation,adhesion and cytoskeletal extension.Group A presents the least.While group B is between the two groups.All the comparisons have significant statistic differences.In this way,ultrasonic micro-arc oxidized pure magnesium-phytic acidsalvianic acid A sodium composite layer can prominently improve the cellular biocompatibility by enhancing cell adhesion and proliferation.Conclusions : The composite material layer with ultrasonic micro-arc oxidized pure magnesium-phytic acid-salvianic acid A sodium presents has the best cellular biocompatibility.