Pure Magnesium Ultrasonic Micro-arc Oxidation(MAO)-phytic Acid-silk Fibroin Composites Research Of Biocompatibility

Objective: The surface of pure magnesium produced micro pores after micro arc oxidation treatment,then closed the pore formed by micro arc oxidationafter through the composite coating which was processed by phytic acid and silk fibroin.Therefore,the adhesion of pure magnesium and material surface is enhanced,and the degradation rate of pure magnesium is further controlled.We explore the biocompatibility of the surface of pure magnesium treated with different coating through detecting the surface coating properties of three different experimental groups.Methods: the experiment was divided into three groups,A group was pure magnesium ultrasonic micro arc oxidation,B group was pure magnesium ultrasonic micro arc oxidationacid,C group was pure magnesium ultrasonic micro arc oxidation-acid-silk fibroin.Surface morphology was observed by SEM,cell proliferation was detected by CCK-8,The activity of alkaline phosphatase was detected by ALP.Results: After MAO treatment,the micro pores and cracks of layer were reduced.and the hydrogen bond and coordination bond between silk fibroin and the hydroxyl group of the acid interacted each other,which was easy to film,and increased the biological activity.Therefore,the C group is better than the B group,the B group is better than the A group in the aspect of cell proliferation,activity,all of them have obvious improvement.Conclusion: the biological compatibility of the test pieces after the complex treatment of theultrasonic micro are oxidation,Phytic acid and Silk Fibroin was significantlyimproved,which was superior to the other two groups.