| Research purposes: in order to further improve the biocompatibility of the magnesium alloy dental implants,Based on pure magnesium micro-arc Oxidation Micro-arc Oxidation(MAO)coating further assembly has protective effect of metal toothy Phytic acid chelating agent,And antimicrobial properties of metal ions,zinc and copper,After implanted into animals,from the perspective of imaging,histological and immunological analysis and evaluation after MAO composite treatment of coating in the pure magnesium the biocompatibility in implants and bone combined effect,Hope can offer shorter implants bone combined with new implant surface treatment method and theoretical basis.Research methods: first,to the pure magnesium implants after micro-arc oxidation in lye(NaOH)in the processing,then have protective effect on the surface of the metal chelating agent phytic acid.To be metal zinc ion and copper ion respectively,the three groups as experimental group,will only by micro-arc oxidation treatment of pure magnesium implant as control group,which is divided into four groups,respectively,for the pure magnesium micro-arc oxidation group MAO as the control group A,MAO-phytic acid treatment as experimental sample,MAO-phytic acid-B of zinc as experimental C,MAO-phytic acid-brass as experimental group D.Before the animal model of implanted with four groups observed by a scanning electron microscope(SEM),respectively,the surface of the implants,to compare and energy spectrum analysis.After the four groups of implants implanted in rabbit mandible,after implantation of 2,4,6 weeks when executed animal models respectively,complete with the mandible bone implant removed,CBCT on 3 d scanning observation implant and bone growth and interface bonding situation;For tissue defect around implants after HE staining,analysis and discuss the change of different groups of various tissues around the implant;At the same time around the implant bone tissue of BMP-2 immunohistochemical observation and analysis.The results of analysis:1.The scanning electron microscopeBefore implantation respectively implants of different coating was observed with sem.the visible pure magnesium MAO coating on the surface of porous morphology,pore size,easy to make cell nutrient solution or corrosion fluid through the porous magnesium substrate.And after dealing with the phytic acid,pore filling,coating pore size smaller.Zinc,copper can be seen after have particles adhere to the surface of phytic acid,play a role in sealing holes.2.The CBCT observationObservation of 2,4,6 weeks after implantation of CBCT images show with implanted into the extension of time,especially the implant-bone around implant interface narrowing of low density image.6 weeks when MAO-phytic acid-contained zinc group and MAO-phytic acid-borne copper low density image around implants are smaller than MAO-phytic acid and MAO group.3.HE staining resultsHE dyeing result shows two weeks when MAO-phytic acid-zinc and MAO-phytic acid-load copper group of fiber cell hyperplasia,hypertrophy in multi-level.MAO-visible phytic acid group magnesium nail and surrounding tissues at the junction of fibrous tissue hyperplasia,and two weeks MAO group granulocyte and fiber tissue density and magnesium nail not seen between inflammatory cells,and macrophages.Around MAO-phytic acid-carrying copper set of fibrous tissue in scattered around the island shape osteogenesis.MAO-phytic acid-contained zinc group of osteoblast around,see a few pink bone matrix.MAO-phytic acid magnesium group nailed almost magnesium in fibrous tissue around the lateral cell hypertrophy,no obvious bone formation.MAO group of fiber cells arranged around the fiber cells loose MAO group two weeks.Six weeks when MAO-phytic acid-visible form around flake copper group osteogenesis,fiber arrangement is neat.Six weeks MAO-phytic acid-group contained zinc magnesium nail forms around a small amount of lamellar bone and visible fibrous cords.MAO-visible phytic acid group around the island development zone,osteoblast layer arranged in the surrounding.Six weeks MAO group of fiber cell hyperplasia obviously visible hypertrophy.4.The immunohistochemical observationBMP-2 immunohistochemical analysis shows on the edge of the six weeks four groups of implants,the strongest positive expression of BMP-2 is the strongest period,promote the osteogenesis ability of MAO-phytic acid-zinc and MAO-phytic acid-load copper group expression are better than MAO-phytic acid group,MAO-phytic acid group times,MAO group the weakest.Conclusion: In pure magnesium surface obtained by micro-arc oxidation technology after disposal process of composite membrane layer can improve on the surface of the implant surface bioactivity,pure magnesium by ultrasound micro-arc oxidation-phytic acid-zinc,copper composite processing,slow slow dissolution of magnesium matrix,and to improve its biocompatibility,has improved the ability to promote the combination of bone more ideal.As on the surface of the implant surface processing method is reliable and promising. |
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